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首页> 外文期刊>Zoological Science >Capacitation and Acrosome Reaction Induction on Thawed Dama dama Deer Spermatozoa: Glycine Effect as Cryopreservation Diluent Supplement
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Capacitation and Acrosome Reaction Induction on Thawed Dama dama Deer Spermatozoa: Glycine Effect as Cryopreservation Diluent Supplement

机译：解冻的达马黄鹿鹿精子的获能和顶体反应诱导：甘氨酸作用作为冷冻保存稀释剂的补充

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摘要

The aim of this research was to evaluate two different diluents for sperm cryopreservation and to study functional parameters in relation to the response to heparin, lysophosphatidylcholine and progesterone, in frozen-thawed semen of fallow deer (Dama dama) during the reproductive season (brama). In this way, fallow deer can be used as a biological model of endangered cervids. Semen was obtained by electroejaculation. Heparin, progesterone and lysophosphatidylcholine were used as capacitation and acrosome reaction inducers, respectively. Capacitation and acrosome reaction were evaluated by chlorotetracycline epifluorescence technique (CTC), membrane integrity by Hypo-osmotic swelling test (HOS) and viability and acrosome integrity by trypan blue stain/DIC. Data was analyzed by ANOVA and Tukey Test (P < 0.05). Semen was cryopreserved in different diluents and Fructose-Tris-Glycine extender was selected. Capacitation with heparin at different incubation times determined that the highest capacitation percentage was obtained at 45 minutes incubation. Progesterone (1 mu M) and lysophosphatidylcholine in heparin capacitated sperm induced acrosome reaction (P < 0.05). This study contributes to improve cryopreservation methods and to increase the knowledge about capacitation and acrosome reaction in vitro in deer spermatozoa, allowing an advance in the development of reproductive biotechnologies.
机译：这项研究的目的是评估冷冻繁殖的小鹿精液在冷冻时精子冷冻保存的两种不同稀释剂，并研究与肝素，溶血磷脂酰胆碱和孕酮反应有关的功能参数。 。这样，小鹿可用作濒临灭绝的子宫颈的生物学模型。通过电射精获得精液。肝素，孕酮和溶血磷脂酰胆碱分别用作获能和顶体反应诱导剂。通过氯四环素表观荧光技术（CTC）评估了获能和顶体反应，通过次渗透溶胀试验（HOS）评估了膜的完整性，并通过台盼蓝染色/ DIC评估了活力和顶体的完整性。数据通过方差分析和Tukey检验进行分析（P <0.05）。将精液冷冻保存在不同的稀释剂中，并选择了果糖-Tris-甘氨酸补充剂。在不同的孵育时间下用肝素进行的获能确定在孵育45分钟时获得了最高的获能百分比。肝素吞噬的精子中的孕酮（1μM）和溶血磷脂酰胆碱引起顶体反应（P <0.05）。这项研究有助于改善冷冻保存方法，并增加有关鹿精子体外获能和顶体反应的知识，从而促进生殖生物技术的发展。

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