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首页> 外文期刊>Zoological Science >Molecular Cloning of Ghrelin and Characteristics of Ghrelin-Producing Cells in the Gastrointestinal Tract of the Common Marmoset (Callithrix jacchus)
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Molecular Cloning of Ghrelin and Characteristics of Ghrelin-Producing Cells in the Gastrointestinal Tract of the Common Marmoset (Callithrix jacchus)

机译：普通Mar猴（Callithrix jacchus）胃肠道中Ghrelin的分子克隆和Ghrelin产生细胞的特征

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摘要

Ghrelin was first isolated from human and rat as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R). In the present study, we determined the ghrelin cDNA sequence of the common marmoset (Callithrix jacchus), a small-bodied New World monkey, and investigated the distribution of ghrelin-producing cells in the gastrointestinal tract and localization profiles with somatostatin-producing cells. The marmoset ghrelin cDNA coding region was 354 base pairs, and showed high homology to that in human, rhesus monkey, and mouse. Marmoset ghrelin consists of 28 amino acids, and the N-terminal region is highly conserved as found in other mammalian species. Marmoset preproghrelin and mature ghrelin have 86.3% and 92.9% homology, respectively, to their human counterparts. Quantitative RT-PCR analysis showed that marmoset ghrelin mRNA is highly expressed in the stomach, but it is not detected in other tissues of the gastrointestinal tract. In addition, a large number of ghrelin mRNA-expressing cells and ghrelin-immunopositive cells were detected in the mucosal layer of the stomach, but not in the myenteric plexus. Moreover, all the ghrelin cells examined in the stomach were observed to be closed-type. Double staining showed that somatostatin-immunopositive cells were not co-localized with ghrelin-producing cells; however, a subset of somatostatin-immunopositive cells is directly adjacent to ghrelin-immunopositive cells. These findings suggest that the distribution of ghrelin cells in marmoset differs from that in rodents, and thus the marmoset may be a more useful model for the translational study of ghrelin in primates. In conclusion, we have clarified the expression and cell distribution of ghrelin in marmoset, which may represent a useful model in translational study.
机译：Ghrelin首先从人和大鼠中分离出来，作为生长激素促分泌素受体（GHS-R）的内源性配体。在本研究中，我们确定了小猿新世界猴-普通mo猴（Callithrix jacchus）的ghrelin cDNA序列，并研究了生长激素释放细胞在胃肠道中的分布以及生长抑素产生细胞的定位特征。 mar猴ghrelin cDNA编码区为354个碱基对，与人，恒河猴和小鼠的同源性很高。 mo猴生长素释放肽由28个氨基酸组成，并且N末端区域的保守性很高，与其他哺乳动物物种一样。 mo猴的前proghrelin和成熟的ghrelin与人类的同源性分别为86.3％和92.9％。定量RT-PCR分析表明，mar猴生长素释放肽mRNA在胃中高表达，但在胃肠道的其他组织中未检测到。另外，在胃的粘膜层中检测到大量表达ghrelin mRNA的细胞和ghrelin免疫阳性细胞，但在肌间神经丛中未检测到。此外，观察到在胃中检查的所有生长素释放肽细胞均为封闭型。双重染色表明，生长抑素免疫阳性细胞与生长素释放肽的细胞并不共定位。然而，生长抑素免疫阳性细胞的一部分直接与生长素释放肽免疫阳性细胞相邻。这些发现表明，生长激素释放肽细胞在mar猴中的分布与在啮齿动物中的分布不同，因此the猴可能是生长素释放肽在灵长类动物中翻译研究的更有用的模型。总之，我们已经阐明了生长激素释放肽在mar猴中的表达和细胞分布，这可能代表了翻译研究中的有用模型。

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