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首页> 外文期刊>Zoological Science >Regulation of on transport in eel intestine by the homologous guanylin family of peptides
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Regulation of on transport in eel intestine by the homologous guanylin family of peptides

机译：同源鸟苷肽家族对鳗肠运输的调节

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摘要

Since the gene expression of guanylin peptides and their receptors, guanylyl cyclase Cs, is enhanced in the intestine of seawater (SW)-adapted eels compared with fresh water (FW)-adapted fish, the guanylin family may play an important role in SW adaptation in eels. The present study analyzed the effect of three homologous guanylin peptides, guanylin, uroguanylin and renoguanylin, on ion movement through the eel intestine, and examined the target of guanylin action using Ussing chambers. The middle and posterior parts of the intestine, where water and ion absorption occurs actively in SW eels, exhibited serosa-negative transepithelial potential, while the anterior intestine was serosa-positive. Mucosal application of each guanylin in the middle or posterior intestine reduced the short-circuit current (I-sc) dose dependently and reversed it at high doses, and reduced electric tissue resistance. The effects were greater in the middle intestine than in the posterior intestine. All three guanylins showed similar potency in the middle segment, but guanylin was more potent in the posterior segment. 8-bromo cGMP mimicked the effect of guanylins. The intestinal response to guanylin was smaller in FW eels. The mucosal presence of NPPB utilized as a CFTR blocker, but not of other inhibitors of the channels/transporters localized on the luminal surface in SW fish intestine, inhibited the guanylin-induced decrease in I-sc. In eels, therefore, the guanylin family may be involved in osmoregulation by the intestine by binding to the receptors and activating CFTR-like channels on the mucosal side through cGMP production, perhaps resulting in Cl- and HCO3- secretion into the lumen.
机译：由于与适应淡水（FW）的鱼类相比，适应海水（SW）的鳗鱼肠中鸟苷肽肽及其受体的基因表达，鸟苷酰环化酶Cs增强，因此鸟苷蛋白家族可能在适应SW在鳗鱼中。本研究分析了三种同源鸟苷肽，鸟苷，尿鸟苷和肾上腺鸟苷对鳗鱼肠中离子迁移的影响，并使用Ussing室检查了鸟苷肽作用的目标。肠道中部和后部在SW鳗中活跃地吸收水分和离子，表现出浆膜阴性的上皮电位，而前肠呈浆膜阳性。在中肠或后肠中每个鸟苷蛋白的粘膜应用可减少剂量的短路电流（I-sc），并在高剂量时将其逆转，从而降低了组织电阻。在中肠的作用大于在后肠的作用。所有三种鸟苷蛋白在中段均显示相似的效价，但鸟苷在后段更有效。 8-溴cGMP模仿鸟苷的作用。 FW鳗鱼对鸟嘌呤的肠道反应较小。 NPPB的粘膜存在被用作CFTR阻滞剂，但不存在于SW鱼肠腔表面上的其他通道/转运蛋白抑制剂，抑制了鸟苷蛋白诱导的I-sc降低。因此，在鳗鱼中，鸟苷蛋白家族可通过与受体结合并通过cGMP产生激活粘膜侧的CFTR样通道，从而参与肠道渗透调节，可能导致Cl-和HCO3-分泌到管腔中。

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