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首页> 外文期刊>Zoological Science >Possible involvement of brain-derived neurotrophic factor (BDNF) in the innervation of dopaminergic neurons from the rat periventricular nucleus to the pars intermedia
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Possible involvement of brain-derived neurotrophic factor (BDNF) in the innervation of dopaminergic neurons from the rat periventricular nucleus to the pars intermedia

机译：脑源性神经营养因子（BDNF）可能参与多巴胺能神经元从大鼠脑室周围核到中间神经的神经支配

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摘要

Developing neurons are guided to their appropriate targets by specific guidance substances that have neurotrophic actions. The aim of the present study was to elucidate the mechanism by which hypothalamic neurons reach the pars intermedia (PI) by correlating the development of dopaminergic (DA) neurons arising in the periventricular nucleus (PeV) of fetal rats with the expression of brain-derived neurotrophic factor (BDNF) in the rat pituitary. The differentiation of DA neurons was observed by immunohistochemistry using an antibody against tyrosine hydroxylase (TH), whereas the ontogenesis of BDNF mRNA in the PI was examined by in situ hybridization and RT-PCR. Immunoreactive TH-neurons were first observed in the PeV at embryonic day (E) 16.5, following which time their axons elongated toward the pituitary. TH-positive reactions were observed in the connective tissue between the PI and the pars nervosa at E20.5. Innervation of the PI by TH-positive neurons was determined at postnatal day (P) 1.5; however, BDNF mRNA was first detected in the PI cells at E175, with an increase in its expression clearly visible at E21.5 and continuing high expression levels in the PI thereafter. These results suggest that BDNF is a specific guidance cue for DA neurons elongating from the PeV to the PI.
机译：发育中的神经元通过具有神经营养作用的特定指导物质被引导至其适当的靶标。本研究的目的是通过将胎鼠脑室周围核（PeV）中产生的多巴胺能（DA）神经元的发育与脑源性表达相关联，阐明下丘脑神经元到达中间pars（PI）的机制。大鼠垂体中的神经营养因子（BDNF）。使用抗酪氨酸羟化酶（TH）的抗体通过免疫组织化学观察到DA神经元的分化，而PI中BDNF mRNA的存在则通过原位杂交和RT-PCR进行了检查。免疫反应性TH神经元首先在PeV胚胎天（E）16.5观察到，此后它们的轴突向垂体拉长。在E20.5时，在PI和神经沟之间的结缔组织中观察到TH阳性反应。在产后第1.5天确定TH阳性神经元对PI的神经支配。然而，BDNF mRNA首先在E175的PI细胞中被检测到，其表达的增加在E21.5处清晰可见，此后在PI中继续保持高表达水平。这些结果表明，BDNF是从PeV到PI延伸的DA神经元的特定指导信号。

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