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首页> 外文期刊>Zoological Science >Morph-Dependent Expression and Subcellular Localization of Host Serine Carboxypeptidase in Bacteriocytes of the Pea Aphid Associated with Degradation of the Endosymbiotic Bacterium Buchnera
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Morph-Dependent Expression and Subcellular Localization of Host Serine Carboxypeptidase in Bacteriocytes of the Pea Aphid Associated with Degradation of the Endosymbiotic Bacterium Buchnera

机译：豌豆蚜虫细菌细胞中宿主丝氨酸羧肽酶的形态依赖性表达和亚细胞定位与内生细菌布氏杆菌的降解有关

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摘要

Pea aphids form a mutualistic association with the endosymbiotic bacterium Buchnera, which is harbored in specialized host cells called bacteriocytes. The adult aphids display dimorphism in which there are winged and wingless morphs. We previously reported that the Buchnera density in bacteriocytes of the winged morph (alate) decreases around final ecdysis, whereas that in the wingless morph (aptera) does not decrease; the decrease in density in alatae is accompanied by activation of the host lysosomal system and by Buchnera degradation. In the present study, we performed a proteomic analysis to clarify the molecular mechanisms underlying the decrease in Buchnera density. By comparing the protein expression profiles of bacteriocytes in alatae and apterae just after final ecdysis, we identified three and one protein spots that were preferentially expressed in alatae and apterae, respectively. Among the three alate-preferential spots, two were an identical aphid protein, carboxypeptidase vitellogenic-like (CPVL), whereas the other was a mixture of four proteins: gamma-glutamyl hydrolase, acyl-CoA dehydrogenase, aphid short chain acyl-CoA dehydrogenase, and Buchnera S-adenosylmethionine synthetase. The aptera-preferential spot was Buchnera outer membrane protein A. Immunoblot and immunohistochemical analyses using aphid bacteriocytes just after final ecdysis revealed that expression of aphid CPVL was preferentially upregulated in alatae and was localized around Buchnera cells in the bacteriocytes, suggesting the involvement of CPVL in Buchnera degradation in alatae.
机译：豌豆蚜虫与内共生细菌Buchnera形成相互联系，后者存在于称为细菌细胞的专门宿主细胞中。成年蚜虫表现出双翅畸形，其中有翅和无翅。我们先前曾报道，有翅变态（alate）的细菌细胞中的布氏杆菌密度在最终蜕皮期附近降低，而无翅变体（aptera）的细菌中的布氏密度没有降低。阿拉特人密度的降低伴随着宿主溶酶体系统的活化和布氏菌的降解。在本研究中，我们进行了蛋白质组学分析，以阐明引起布氏菌密度降低的分子机制。通过比较最终蜕皮后刚毛和and骨中细菌细胞的蛋白质表达谱，我们确定了分别在a毛和骨中优先表达的三个和一个蛋白点。在三个优先的斑点中，两个是相同的蚜虫蛋白，即羧肽酶卵黄样（CPVL），而另一个是四种蛋白的混合物：γ-谷氨酰水解酶，酰基-CoA脱氢酶，蚜虫短链酰基-CoA脱氢酶，和Buchnera S-腺苷甲硫氨酸合成酶。 aptera优先点是布氏杆菌外膜蛋白A。在最终蜕皮后使用蚜虫细菌细胞进行的免疫印迹和免疫组织化学分析显示，蚜虫CPVL的表达在阿拉特地区优先上调，并位于细菌细胞中的布氏杆菌细胞周围，表明CPVL参与了细菌的活动。桦木中的布氏菌降解。

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