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首页> 外文期刊>Zoological Science >Requirement of potassium for the action of anti-diuretic hormone (ADH) on frog skin.
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Requirement of potassium for the action of anti-diuretic hormone (ADH) on frog skin.

机译：钾对青蛙皮肤抗利尿激素（ADH）作用的需要。

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The aim of the present study is to study whether the presence of K(+) in bathing media is required for the action of ADH to the ionic transport across the skin in the frog species Rana hexadactyla. lonic transport was measured as transepithelial potential difference (TEPD) and short circuit current (SCC) by using an indigenously developed computer based voltage-clamp technique. Addition of ADH (40 nM) on the serosal side significantly increased the TEPD and SCC with Normal Ringer (NR) on both sides. ADH had no effect subsequent to amiloride (100 microM) pre-treatment, which confirmed the ADH-induced Na(+) transport. Chloride also has a significant role in the development of TEPD. To determine the role of K(+), Potassium-free Ringer (KFR) was placed on both sides; addition of ADH had no effect consequently. Further experiments were carried out to find out which side of K(+) was required for the action of ADH. There was a lack of ADH effect with apical NR and serosal KFR, demonstrating that serosal K(+) is essential to activate Na(+), K(+)- ATPase. Similarly, the ADH effect was lacking with apical KFR and serosal NR that was the novel finding of this study. Due to the concentration gradient, the K(+) was secreted from serosal side to apical side through barium (1 mM) blockable K(+) channel. This study provides evidence that serosal as well as apical K(+) are necessary for the action of ADH.
机译：本研究的目的是研究在青蛙物种蛙蛙中，ADH对皮肤中离子运输的作用是否需要在沐浴介质中存在K（+）。离子传输通过使用本地开发的基于计算机的电压钳技术测量为上皮电位差（TEPD）和短路电流（SCC）。在浆膜侧添加ADH（40 nM）可以显着增加两侧PDT和SCC，同时具有正常Ringer（NR）。阿米洛利（100 microM）预处理后，ADH无效，这证实了ADH诱导的Na（+）转运。氯化物在TEPD的发展中也起着重要作用。为了确定K（+）的作用，将无钾林格（KFR）置于两侧。因此添加ADH没有作用。进行进一步的实验以发现ADH的作用需要K（+）的哪一侧。顶端NR和浆膜KFR缺乏ADH效应，表明浆膜K（+）对激活Na（+），K（+）-ATPase必不可少。同样地，根尖KFR和浆膜NR缺乏ADH效应，这是本研究的新发现。由于浓度梯度，K（+）通过钡（1 mM）可阻断的K（+）通道从浆膜侧分泌到顶侧。这项研究提供了证据，浆膜以及顶端K（+）是ADH的作用所必需。

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