...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zoological Science >Neuropeptide Y in tiger puffer (Takifugu rubripes): distribution, cloning, characterization, and mRNA expression responses to prandial condition.
【24h】

Neuropeptide Y in tiger puffer (Takifugu rubripes): distribution, cloning, characterization, and mRNA expression responses to prandial condition.

机译：老虎河豚（ Takifugu rubripes ）中的神经肽Y：分布，克隆，特征和对餐后状况的mRNA表达反应。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neuropeptide tyrosine (NPY) is a potent orexigenic neuropeptide implicated in feeding regulation in rodents. However, the involvement of NPY in feeding behavior has not well been studied in fish. Therefore, we investigated the role of NPY in food intake using a tiger puffer (Takifugu rubripes) model. We observed the distribution of NPY-like immunoreactivity in the brain. Neuronal cell bodies containing NPY were located in the telencephalon, hypothalamus, mesencephalon, and medulla oblongata, and their nerve fibers were also found throughout the brain. We cloned two cDNAs, encoding NPYa and NPYb orthologs, respectively, from the brain, and also confirmed two genes encoding these NPYs in the Takifugu genome database. We examined the distribution of these transcripts in the brain using real-time PCR. Levels of NPYa mRNA in the telencephalon, mesencephalon and hypothalamus were much higher than in the medulla oblongata and cerebellum, whereas levels of NPYb mRNA in the medulla oblongata were higher than in other regions. We also examined prandial effects on the expression level of these transcripts in the telencephalon and hypothalamus. NPYa mRNA levels in the hypothalamus, but not in the telencephalon, obtained from fish fasted for one week were higher than those in fish that had been fed normally. The level was decreased at 2 h after feeding. Levels of NPYb mRNA were not affected by prandial conditions. These results suggest that NPY is present throughout the brain, and that NPYa, but not NPYb, in the hypothalamus is involved in the feeding regulation in the tiger puffer.
机译：神经肽酪氨酸（NPY）是一种强力的致病性神经肽，与啮齿动物的进食调节有关。但是，尚未对鱼类中NPY参与进食行为进行深入研究。因此，我们使用老虎河豚（）模型调查了NPY在食物摄入中的作用。我们观察到脑中NPY样免疫反应性的分布。含有NPY的神经元细胞位于远脑，下丘脑，中脑和延髓，它们的神经纤维也遍布整个大脑。我们从大脑中克隆了两个分别编码NPYa和NPYb直系同源基因的cDNA，并在 Takifugu 基因组数据库中确认了两个编码这些NPY的基因。我们使用实时PCR检查了这些转录本在大脑中的分布。端脑，中脑和下丘脑中的NPYb mRNA水平远高于延髓和小脑，而延髓中的NPYb mRNA水平则高于其他地区。我们还检查了餐时对这些蛋白在端脑和下丘脑中表达的影响。从禁食一周的鱼中获取的下丘脑中NPYa mRNA水平（但在末梢脑中则不然）高于正常喂养的鱼中NPYa mRNA水平。喂食后2小时血药浓度降低。 NPYb mRNA的水平不受饮食条件的影响。这些结果表明，NPY存在于整个大脑中，下丘脑中的NPYa但不涉及NPYb参与了河豚的进食调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号