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首页> 外文期刊>Zoological Science >Characterization of an Alternative Chromatin Remodeling to Parasperm in a Cottid Fish, Hemilepidotus gilberti
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Characterization of an Alternative Chromatin Remodeling to Parasperm in a Cottid Fish, Hemilepidotus gilberti

机译：替代染色质重塑成乌头鱼Hemilepidotus gilberti的精子

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摘要

The dimorphic sperm of Hemilepidotus gilberti, i.e., haploid eusperm and diploid parasperm, have different morphologies corresponding to their own roles in fertilization. To estimate how these specific sperm morphologies were established, we focused on the nuclear morphologies and examined their changing processes in dimorphic spermiogenesis. Electron microscopic observation revealed that, in euspermatids, chromatin condensation first appeared as a mosaic pattern of moderate electrodense material in the peripheral region of the round nucleus. Those materials spread across the whole area to form a uniformly condensed nucleus. Chromatin condensation began similarly in paraspermatids to that in euspermatids. These became localized to one side of a nucleus and further condensed to form strong electrodense chromatin clusters, which are a specific feature of parasperm. From the remodeled nuclei of eusperm and parasperm, we found five and three kinds of sperm-specific basic proteins (SBPs), respectively, substituted to histones. The N-terminus amino acid sequences of the SBPs suggest that, in parasperm, one major SBP and two minor ones were distinct from each other. In eusperm nuclei, two kinds of specific SBPs were detected in addition to the homologs of parasperm SBPs. The specific SBPs had homologous amino acid sequences with huge arginine clusters, and one of them was most dominant among the five kinds of SBPs. The different combinations of SBPs in the eusperm and parasperm may cause a specific pattern of chromatin condensation in the dimorphic sperm nuclei of H. gilberti.
机译：吉尔伯氏绣线虫的双态精子，即单倍体胚乳和二倍体副精子，具有不同的形态，分别对应于它们在受精中的作用。为了估计这些特定的精子形态是如何建立的，我们集中研究了核形态，并研究了它们在双态精子发生过程中的变化过程。电子显微镜观察发现，在大隐皮类动物中，染色质凝结首先在圆核的外围区域以中等电极物质的镶嵌图案出现。这些物质散布在整个区域，形成均匀凝聚的核。染色质的凝结在精原虫中的开始与在精原虫中的相似。这些被定位在细胞核的一侧，并进一步凝结形成强电染色质簇，这是精子的一个特殊特征。从被子植物和被子植物的重组核中，我们发现分别有五种和三种精子特异性碱性蛋白（SBPs）被组蛋白取代。 SBP的N末端氨基酸序列表明，在精子中，一个主要的SBP和两个次要的SBP彼此不同。在胚乳核中，除了被子植物SBP的同源物外，还检测到两种特异性SBP。特异的SBP具有同源的氨基酸序列和巨大的精氨酸簇，其中一种在5种SBP中占主导地位。胚乳和精子中SBP的不同组合可能会导致吉尔伯特氏菌双态精子核中染色质浓缩的特定模式。

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