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首页> 外文期刊>Zoological Science >Effects of Endocrine Disruptors on Bombina orientalis P450 Aromatase Activity
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Effects of Endocrine Disruptors on Bombina orientalis P450 Aromatase Activity

机译：内分泌干​​扰物对Bombina Orientalis P450芳香酶活性的影响

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摘要

To assess the effects of endocrine-disrupting chemicals on the expression and activity of aromatase in the gonads of Bombina orientalis, a common amphibian, we intraperitoneally injected nonylphenol or bisphenol-A and then examined aromatase mRNA levels by RT-PCR as well as aromatase enzymatic activity by tritiated water release assays. To design primers for the RT-PCR, we cloned the B. orientalis aromatase gene using RT-PCR and degenerate primers. The full-length cDNA was obtained by 5'- and 3'-RACE PCR. The complete sequence of the B. orientalis aromatase gene revealed an open reading frame of 1500 bp encoding a deduced protein of 500 amino acids. Semi-quantitative RT-PCR indicated that nonylphenol or bisphenol-A injection did not significantly affect the expression of B. orientalis aromatase mRNA. However, a 48-hr treatment with nonyphenol or bisphenol A reduced aromatase activity to 47% and 32% of the control, respectively. These results suggest that endocrine disruptors can effectively modulate the activity of B. orientalis aromatase without affecting its mRNA levels.
机译：为了评估破坏内分泌的化学物质对东方两栖动物Bombina Orientalis性腺中芳香化酶表达和活性的影响，我们腹膜内注射壬基酚或双酚A，然后通过RT-PCR和芳香化酶检测芳香化酶mRNA的水平。通过ti水释放试验测定其活性。为了设计用于RT-PCR的引物，我们使用RT-PCR克隆了东方双歧杆菌芳香酶基因并进行简并引物。通过5'-和3'-RACE PCR获得全长cDNA。东方芽孢杆菌芳香酶基因的完整序列揭示了一个1500 bp的开放阅读框，编码了500个氨基酸的推导蛋白。半定量RT-PCR表明，壬基酚或双酚A注射液不会显着影响东方双歧杆菌芳香酶mRNA的表达。但是，用壬基酚或双酚A处理48小时后，芳香化酶活性分别降低至对照的47％和32％。这些结果表明，内分泌干扰物可以有效地调节东方芽孢杆菌芳香酶的活性，而不会影响其mRNA水平。

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