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首页> 外文期刊>Zoological Science >Sodium Accumulation in Decomposing Yolk Platelets during the Development of Xenopus Iaevis
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Sodium Accumulation in Decomposing Yolk Platelets during the Development of Xenopus Iaevis

机译：非洲爪蟾发育过程中分解卵黄血小板中的钠积累。

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To cytochemically demonstrate the accumulation of sodium ions in decomposing yolk platelets, we first aimed to develop a method of sodium detection by administrating the magnesium uranyl acetate (double acetate) reagent, which throws down the sodium as triple acetate, to sciatic nerves of Xenopus laevis. By observing the cell contour and the volume of the precipitates produced in the intercellular and interlamellar spaces of the myelin sheath around the nerves, we determined that a 15-times dilution of the original double acetate reagent (Caley and Foulk, 1929) was the best concentration for biological usage. The validity of the double acetate method was assessed by observing the specific localization of triple acetate precipitates in the skeletal muscle and kidney;l In addition to their localization in intercellular spaces, the precipitates were preferentially present in the transverse system of skeletal muscle cells and present in the ground cytoplasm as well as in the organelles other than vacuoles of proximal convoluted tubule cells of the kidney. By applying this method to developing embryos, it was found that the sodium ions are stored in the vesicles during the cleavage stages of development, are apparently transported by the vesicles to decomposing yolk platelets at the early neural plate and later stages, and are accumulated in these platelets. Those results would satisfy the prediction that the sodium concentration must be increased in decomposing yolk platelets, since yolk solubilization by high salt concentrations is prerequisite for amphibian yolk digestion to occur.
机译：为了通过细胞化学方法证明钠离子在卵黄质血小板中的积累，我们的首要目标是开发一种检测钠的方法，方法是将乙酸铀酰乙酸镁（双乙酸酯）试剂投掷到非洲爪蟾的坐骨神经上，从而将钠以三乙酸酯的形式投下。 。通过观察细胞轮廓以及在神经周围髓鞘的细胞间和层间空间中产生的沉淀物的体积，我们确定原始双乙酸盐试剂（Caley和Foulk，1929年）的15倍稀释是最好的。生物使用浓度。通过观察三乙酸盐沉淀物在骨骼肌和肾脏中的特定定位来评估双乙酸盐方法的有效性; l除了其在细胞间隙中的定位外，这些沉淀物优先存在于骨骼肌细胞的横向系统中，并且存在在肾的近端回旋小管细胞的液泡中，在地下细胞质中以及在细胞器中。通过将这种方法应用于发育中的胚胎，发现钠离子在发育的卵裂期被储存在囊泡中，显然在早期的神经板和后期被囊泡转运至分解卵黄质的血小板，并在卵泡中积累。这些血小板。这些结果将满足以下预测：在分解卵黄血小板时必须增加钠浓度，因为高盐浓度的卵黄溶解是两栖卵黄消化发生的前提。

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