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首页> 外文期刊>Zoological Science >Comparative Transcriptomic Analysis of Two Closely Related Ground Beetle Species with Marked Genital Divergence Using Pyrosequencing
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Comparative Transcriptomic Analysis of Two Closely Related Ground Beetle Species with Marked Genital Divergence Using Pyrosequencing

机译：利用焦磷酸测序技术比较两个显着生殖器差异的近缘甲壳虫物种的转录组学分析

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摘要

Ground beetles of the subgenus Ohomopterus (genus Carabus) show marked divergence in species-specific male and female genital morphologies, which contributes to reproductive isolation among species. Characterizing the genetic basis of species-specific genital morphology is essential for understanding their diversification, but genomic information on Ohomopterus is not yet available. We analyzed mRNA extracted from abdominal sections of the last instar larvae and pupae of two sister species, Carabus (Ohomopterus) iwawakianus and C. (O.) uenoi, which show marked differences in genital morphology, to compare transcriptomic profiles using Roche 454 pyrosequencing. We obtained 1,608,572 high-quality reads and assembled them into 176,278 unique sequences, of which 66,049 sequences were combined into 12,662 clusters. Differential expression analyses for sexed pupae suggested that four and five clusters were differentially expressed between species for males and females, respectively. We also identified orthologous sequences of genes involved in genital development in Drosophila, which potentially affect genital development and species-specific genital morphology in Ohomopterus. This study provides the first large transcriptomic data set for a morphologically diversified beetle group, which can facilitate future studies on the genetic basis of species-specific genitalia.
机译：Ohomopterus（Carabus属）的地面甲虫在特定物种的男性和女性生殖器形态上表现出明显的差异，这有助于物种间的生殖隔离。表征特定物种生殖器形态的遗传基础对于理解其多样性至关重要，但是关于单翅目的基因组信息尚不可用。我们分析了从两个姐妹物种Carabus（Ohomopterus）iwawakianus和C.（O.）uenoi的最后一个幼虫幼虫和p的腹部提取的mRNA，以显示生殖器形态上的显着差异，以比较使用Roche 454焦磷酸测序的转录组谱。我们获得了1,608,572个高质量读段，并将它们组装成176,278个独特序列，其中66,049个序列组合成12,662个簇。对性up的差异表达分析表明，雄性和雌性物种之间分别有四个和五个簇差异表达。我们还确定了果蝇生殖器发育中涉及的基因的直系同源序列，这些序列可能影响单翅目的生殖器发育和物种特异性生殖器形态。这项研究提供了形态上多样化的甲虫组的第一个大型转录组数据集，这可以促进将来基于物种特异性生殖器的遗传学研究。

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