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首页> 外文期刊>Zoological Science >Sea Lily Muscle Lacks a Troponin-Regulatory System, While It Contains Paramyosin
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Sea Lily Muscle Lacks a Troponin-Regulatory System, While It Contains Paramyosin

机译：海上百合肌肉缺乏肌钙蛋白调节系统，虽然它包含副肌球蛋白。

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Troponin, a Ca2+-dependent regulator of striated muscle contraction, has been characterized in vertebrates, protochordates (amphioxus and ascidian), and many invertebrate animals that are categorized in protostomes, but it has not been detected in echinoderms, such as sea urchin and sea cucumber, members of subphylum Eleutherozoa. In this study, we examined the muscle of a species of isocrinid sea lilies, a member of subphylum Pelmatozoa, that constitute the most basal group of extant echinoderms to clarify whether troponin is lacking from the early evolution of echinoderms. Native thin filaments were released from the muscle homogenates in a relaxing buffer containing ATP and EGTA, a Ca2+-chelator, and were collected by ultra-centrifugation. Actin and tropomyosin, but not a troponin-like protein, were detected in the filament preparation. The filaments increased Mg2+-ATPase activity of rabbit skeletal muscle myosin irrespective of the presence or absence of Ca2+. The results indicate that Ca2+-sensitive factor, troponin, is lacking in the thin filaments of sea lily muscle as in those of the other echinoderms, sea urchin and sea cucumber. On the other hand, a paramyosin-like protein that is absent from chordates was detected in sea lily muscle as in the muscles of the other echinoderms and invertebrate animals of protostomes.
机译：肌钙蛋白是横纹肌收缩的一种依赖Ca2 +的调节剂，已在脊椎动物，原生动物（amphioxus和ascidian）以及许多无脊椎动物中被归类为原生动物，但尚未在棘皮动物如海胆和海中发现。黄瓜，隶属叶锦紫苏。在这项研究中，我们检查了异旋毛虫海百合属的一个物种，即佩尔马图亚亚属的一种肌肉，它们构成现存的棘皮动物的最基础群体，以阐明从棘皮动物的早期进化中是否缺乏肌钙蛋白。在含有ATP和EGTA（一种Ca2 +螯合剂）的松弛缓冲液中，从肌肉匀浆中释放天然细丝，并通过超速离心收集。在长丝制品中检测到肌动蛋白和原肌球蛋白，但未检测到肌钙蛋白样蛋白。不论是否存在Ca2 +，这些细丝都会增加兔骨骼肌肌球蛋白的Mg2 + -ATPase活性。结果表明，与其他棘皮动物，海胆和海参一样，海百合肌肉细丝中缺乏钙离子敏感因子肌钙蛋白。另一方面，在海百合肌肉中，与其他棘皮动物和无脊椎动物的无脊椎动物动物的肌肉中一样，在脊索动物中检测到了类除肌球蛋白样蛋白。

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