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首页> 外文期刊>Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz >Development of UV-induced Carbendazim-resistant mutants of Trichoderma harzianum for integrated control of damping-off disease of cotton caused by Rhizoctonia solani
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Development of UV-induced Carbendazim-resistant mutants of Trichoderma harzianum for integrated control of damping-off disease of cotton caused by Rhizoctonia solani

机译：紫外线诱导的哈茨木霉多菌灵抗性突变体的开发，用于综合控制日光梭菌引起的棉花减毒病

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摘要

The treatment of seeds with Trichoderma harzianum has been used successfully to control damping-off of cotton and other crops. However, T harzianum is very sensitive to carbendazim which is often used as a seed-dressing fungicide for the control of internally seed-borne fungal pathogens. Hence in the present study, carbendazim-resistant mutants of T harzianum were developed through UV-irradiation. These mutants grew well in PDA medium containing the carbendazim even at 100 mug/ml. Sustained production of cellulase and chitinase by these mutants in the presence of carbendazim in the growth medium was observed. These mutants established well in the rhizosphere of cotton plants. Seed treatment with carbendazim followed by application of carbendazim-resistant mutants of T harzianum resulted in better plant stand, plant biomass and less damping-off disease caused by R. solani in both greenhouse and field conditions.
机译：用哈茨木霉处理种子已成功用于控制棉花和其他农作物的减湿。但是，塔哈木对多菌灵非常敏感，多菌灵通常被用作拌种杀真菌剂，以控制内部种子传播的真菌病原体。因此，在本研究中，通过紫外线照射开发了抗多菌灵的哈茨木霉突变体。这些突变体在含有多菌灵的PDA培养基中生长良好，即使浓度为100杯/毫升。在多菌灵存在下，在生长培养基中观察到这些突变体持续产生纤维素酶和几丁质酶。这些突变体在棉花植物的根际中建立良好。用多菌灵进行种子处理，然后应用抗多菌灵的哈尔茨木霉突变体，可在温室和田间条件下改善植株生长，提高植物生物量，并减少由茄红枯菌引起的减毒病。

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