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首页> 外文期刊>Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz >Antagonistic potential of Thchoderma spp. against Rhizoctonia solani and use of M13 microsatellite-primed PCR to evaluate the antagonist genetic variation
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Antagonistic potential of Thchoderma spp. against Rhizoctonia solani and use of M13 microsatellite-primed PCR to evaluate the antagonist genetic variation

机译：木霉菌的拮抗潜力。抗茄枯萎病菌并利用M13微卫星引物PCR评价拮抗剂的遗传变异

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摘要

Rhizoctonia solani is the most important pathogen involved in cotton seedling disease in Egypt-Variations in the antagonistic activity of 20 isolates belonging to Trichoderma harzianum and 7, longi-brachiatum were evaluated in vitro, against three isolates of Rhizoctonia solani in dual culture There was highly significant interaction between isolates of Trichoderma and R solani. All isolates of Trichodetrma spp showed varying levels of antagonism against R. solani. Similarly, the interaction betweenTricho-derma isolates and R solani was a highly significant source of variation in surviving seedlings under greenhouse conditions. In vitro and in vivo antagonism were not correlated, which indicated that, in vitro inhibition of the pathogen was not predictive of in vivo biocontrol by an individual antagonist Microsatellite-primed PCR analysis was used to evaluate the genetic variation among Trichoderma isolates The analysis divided the isolates into two main groups. Grouping the isolates based on cluster analysis of their DNA profiles matched that based on their morphological taxonomy. However, no congmency was found between cluster analyses obtained by PCR and cluster analysis by efficiency parameters of the biocontiol agents.
机译：solani solani是埃及棉苗病害中最重要的病原体。对哈茨木霉（Trichoderma harzianum）和7 longi-brachiatum的20株菌株的体外拮抗活性进行了评估，在双重培养中对3株solani solani具有拮抗活性。木霉和R solani的分离株之间的显着相互作用。 Trichodetrma spp的所有分离株均表现出不同的拮抗R. solani的能力。同样，在温室条件下，Tricho-derma分离株和R solani之间的相互作用是存活的幼苗中高度重要的变异来源。体外和体内拮抗作用没有相关性，这表明，单个病原体的体外抑制作用不能预测通过单个拮抗剂进行的体内生物控制。微卫星引物PCR分析用于评估木霉分离物之间的遗传变异。分为两个主要类别。根据其DNA谱的聚类分析对分离株进行分组与基于其形态分类学的分离株匹配。然而，在通过PCR获得的聚类分析和通过生物contiol剂的效率参数的聚类分析之间没有发现一致性。

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