Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater

Marcelino I; Vachiery N; Amaral AI; Roldao A; Lefrancois T; Carrondo MJ; Alves PM; Martinez D

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Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater

机译：纯化工艺和储存条件对灭活心水疫苗效果的影响

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This work evaluates the effect of purification process and storage conditions (buffer formulation and temperature) on the efficacy of Ehrlichia ruminantium (ER) elementary bodies to be used as an inactivated vaccine candidate against heartwater. In vitro assays revealed that, to avoid major losses in ER integrity and corresponding antigenic properties, a buffer with pH between 5.6 and 8 and an osmolality above 100 mOsmol/kg H(2)O is recommended. Amongst the tested formulations, both PBS and NaCl have shown to stabilize ER antigens at -20 degrees C. To assess the protective properties of the different vaccine formulations, in vivo experiments were performed using a goat model. The results obtained showed that the preparation of ER antigens using a novel membrane-based purification strategy and a simple vaccine formulation (NaCl, -20 degrees C) induced equivalent protection to the conventional vaccine based on ER antigens prepared by a multistep centrifugation methodology and stored at -20 degrees C in PBS buffer.

机译：这项工作评估了纯化过程和储存条件（缓冲液配方和温度）对反刍动物毛虫（ER）基本体功效的影响，这些物质可用作灭活心水的候选疫苗。体外试验表明，为避免ER完整性和相应抗原特性的重大损失，建议使用pH值介于5.6和8之间且重量摩尔渗透压浓度高于100 mOsmol / kg H（2）O的缓冲液。在测试的制剂中，PBS和NaCl均显示可在-20摄氏度下稳定ER抗原。为评估不同疫苗制剂的保护特性，使用山羊模型进行了体内实验。获得的结果表明，使用基于膜的新型纯化策略和简单的疫苗制剂（NaCl，-20摄氏度）制备ER抗原，与通过多步离心方法制备并储存的基于ER抗原的常规疫苗具有相同的保护作用。在-20摄氏度的PBS缓冲液中。

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《Vaccine》 |2007年第26期|共11页
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Marcelino I; Vachiery N; Amaral AI; Roldao A; Lefrancois T; Carrondo MJ; Alves PM; Martinez D;
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正文语种 eng
中图分类卫生标准、卫生检查、医药管理;
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1. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater [J] . Marcelino I, Vachiery N, Amaral AI, Vaccine . 2007,第26期

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Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater

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