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首页> 外文期刊>Vaccine >Pluronic F127 enhances the effect as an adjuvant of chitosan microspheres in the intranasal delivery of Bordetella bronchiseptica antigens containing dermonecrotoxin
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Pluronic F127 enhances the effect as an adjuvant of chitosan microspheres in the intranasal delivery of Bordetella bronchiseptica antigens containing dermonecrotoxin

机译：Pluronic F127增强了壳聚糖微球佐剂在鼻内递送含皮肤坏死毒素的支气管败血博德氏杆菌抗原中的作用

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摘要

We have studied a vaccine delivery system in vitro and in vivo based on chitosan microspheres (CMs) prepared in the presence of selected immunomodulators, Pluronic block copolymer F127 (F127). The Bordetella bronchiseptica multiple antigens containing dermonecrotoxin (BBD), a virulent factor leading to atrophic rhinitis (AR) in swine was loaded in CMs/F127 or CMs alone. The microspheres, prepared using an ionic gelation process with tripolyphosphate, demonstrated release profiles that showed a greater amount of BBD being released from BBD-loaded CMs/F127 (BBD-CMs/F127). In vitro experiments using mouse alveolar macrophage cells (RAW 264.7) demonstrated that BBD-CMs/F127 have significantly higher immune-stimulating activities than controls. The highest immune-stimulating activities by the BBD-CMs/F127 using RAW 264.7 cells were mirrored in the in vivo studies following nasal administration to mice. The mice immunized with BBD-CMs/F127 showed higher BBD specific IgA antibody responses in nasal wash, saliva and serum than mice immunized with BBD-CMs alone. Protective immunity was measured by survival rate after challenge with B. bronchiseptica via the nasal cavity. The survival rate of the group treated with BBD-CMs/F127 was higher than those of other groups. These results suggested that CMs/F127 represents a novel mucosal delivery system and that F127 could enhance the delivery of BBD-CMs in the vaccination scheme.
机译：我们已经研究了基于壳聚糖微球（CMs）在体外和体内的疫苗输送系统，该壳聚糖是在选定的免疫调节剂Pluronic嵌段共聚物F127（F127）存在下制备的。含有皮肤坏死毒素（BBD）的支气管败血博德特氏菌多种抗原，是猪中导致萎缩性鼻炎（AR）的一种毒性因子，被单独装入CM / F127或CM中。使用三聚磷酸盐的离子胶凝工艺制备的微球具有良好的释放特性，表明释放出大量BBD的是BBD负载的CMs / F127（BBD-CMs / F127）。使用小鼠肺泡巨噬细胞（RAW 264.7）进行的体外实验表明，BBD-CMs / F127具有比对照组更高的免疫刺激活性。在鼻腔给药给小鼠后的体内研究中，BBD-CMs / F127使用RAW 264.7细胞具有最高的免疫刺激活性。与单独用BBD-CMs免疫的小鼠相比，用BBD-CMs / F127免疫的小鼠在鼻洗液，唾液和血清中显示出更高的BBD特异性IgA抗体反应。通过经由鼻腔用支气管败血性博德氏菌攻击后的存活率来测量保护性免疫。 BBD-CMs / F127治疗组的生存率高于其他组。这些结果表明，CMs / F127代表了一种新型的粘膜递送系统，F127可以在疫苗接种方案中增强BBD-CM的递送。

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