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首页> 外文期刊>Vaccine >Use of the stationary phase inducible promoters, spv and dps, to driveheterologous antigen expression in Salmonella vaccine strains
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Use of the stationary phase inducible promoters, spv and dps, to driveheterologous antigen expression in Salmonella vaccine strains

机译：固定相诱导型启动子spv和dps在沙门氏菌疫苗株中驱动异源抗原表达的用途

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摘要

We have investigated the ability of the growth phase regulated promoters dps and spy, to drive expression of heterologous antigens in Salmonella vaccine strains. Reporter plasmids were constructed which directed P-galactosidase expression from rips (pDpslacZ) or spv (pSpvlacZ) and these were introduced independently into the Salmonella typhimurium vaccine strain SL3261 (aroA(-)). beta-galactosidase expression was induced 20-fold and 100-fold when broth cultures of SL3261 (pDpslacZ) or SL3261 (pSpvlacZ) respectively, entered the stationary phase of growth. Within macrophages, P-galactosidase expression was induced 3.5-fold with SL3261 (pDpslacZ) and 7-fold with SL3261 (pSpvlacZ). The spv and dps promoters were used to drive independent expression of the C fragment domain of tetanus toxin (TetC) from plasmids harboured in S. typhimurium SL3261. Levels of anti-TetC antibodies were significantly higher in the sera of BALB/c mice perorally inoculated with SL3261 (pSpvtetC) or SL3261 (pDpstetC) compared to unvaccinated controls. This suggests that these promoter systems may be used to drive foreign antigen expression in live oral Salmonella vaccines.
机译：我们已经研究了生长期调节的启动子dps和spy的能力，以驱动沙门氏菌疫苗株中异源抗原的表达。构建了报告质粒，其指导来自裂片（pDpslacZ）或spv（pSpvlacZ）的P-半乳糖苷酶表达，并将它们独立地引入鼠伤寒沙门氏菌疫苗菌株SL3261（aroA（-））。当SL3261（pDpslacZ）或SL3261（pSpvlacZ）的肉汤培养分别进入生长的稳定期时，β-半乳糖苷酶的表达被诱导20倍和100倍。在巨噬细胞中，P-半乳糖苷酶表达被SL3261（pDpslacZ）诱导3.5倍，被SL3261（pSpvlacZ）诱导7倍。 spv和dps启动子用于驱动鼠伤寒沙门氏菌SL3261中携带的质粒的破伤风毒素（TetC）C片段结构域独立表达。与未接种疫苗的对照组相比，经口接种SL3261（pSpvtetC）或SL3261（pDpstetC）的BALB / c小鼠血清中的抗TetC抗体水平明显更高。这表明这些启动子系统可用于驱动活的口服沙门氏菌疫苗中的外源抗原表达。

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