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首页> 外文期刊>Vaccine >Prime-boost vaccination using cysteine proteinases type I and II of Leishmania infantum confers protective immunity in murine visceral leishmaniasis
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Prime-boost vaccination using cysteine proteinases type I and II of Leishmania infantum confers protective immunity in murine visceral leishmaniasis

机译：使用婴儿利什曼原虫I型和II型半胱氨酸蛋白酶的初免-加强疫苗接种可在小鼠内脏利什曼病中提供保护性免疫

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摘要

Vaccination with a cocktail of DNA encoding cysteine proteinases has been previously shown to confer protection against experimental cutaneous leishmaniasis (CL). In the present study we test the efficacy of immunization against Leishmania infantum in a murine model of infection, using a prime-boost strategy. BALB/c mice were immunized twice, in a 3 weeks interval, with cocktail of plasmids DNA encoding type I (cpb) and II (cpa) cysteine proteinases. DNA immunization was then followed by a boost with rCPA/rCPB in addition to CpG ODN and Montanide720 as adjuvant. Analysis of the immune response showed that vaccination mainly elicited antigen-specific IgG2a antibodies, suggesting the induction of a Th1 immune response. This was further confirmed by the analysis of the splenic cytokine production: at all time points the ratio of IFN-gamma/IL-5 induced upon restimulation with rCPA and rCPB was always significantly higher in vaccinated group compared to both control groups.
机译：先前已证明，用编码半胱氨酸蛋白酶的DNA混合物进行疫苗接种可赋予针对实验性皮肤利什曼病（CL）的保护。在本研究中，我们使用初免-加强策略在小鼠感染模型中测试了针对婴儿利什曼原虫的免疫效果。在3周的间隔内，用编码I型（cpb）和II型（cpa）半胱氨酸蛋白酶的质粒DNA混合物对BALB / c小鼠免疫两次。 DNA免疫后，除了使用CpG ODN和Montanide720作为佐剂外，还使用rCPA / rCPB加强免疫。对免疫反应的分析表明，疫苗接种主要引发了抗原特异性IgG2a抗体，提示了Th1免疫反应的诱导。通过脾脏细胞因子产生的分析进一步证实了这一点：在所有时间点，接种疫苗的组中，rCPA和rCPB再刺激后诱导的IFN-γ/ IL-5的比例始终比两个对照组都高得多。

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