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首页> 外文期刊>Vaccine >Comparative efficacy of an indigenous 'inactivated vaccine' using highly pathogenic field strain of Mycobacterium avium subspecies paratuberculosis 'Bison type' with a commercial vaccine for the control of Capri-paratuberculosis in India
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Comparative efficacy of an indigenous 'inactivated vaccine' using highly pathogenic field strain of Mycobacterium avium subspecies paratuberculosis 'Bison type' with a commercial vaccine for the control of Capri-paratuberculosis in India

机译：使用高致病性禽分枝杆菌亚种副结核病“野牛型”与商业疫苗在印度控制卡普里副结核病的本地“灭活疫苗”的比较功效

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Johne's disease (JD) is endemic in goatherds located at Central Institute for Research on Goats, Makhdoom, since 1979 and lately it has been reported from farmer's herds in equal frequencies. Despite using test and slaughter method for the control of JD for more than 25 years in these herds, incidence of JD has not been reduced. Efficacy of 'indigenous vaccine' containing native 'Bison type' genotype of Mycobacterium avium subspecies paratuberculosis (MAP) was compared with commercial vaccine using challenge studies with homologous strain of MAP. Goat kids (85) were randomly divided in to three groups. Kids were vaccinated with 1ml of vaccine subcutaneously and Sham-immunized with 1ml of sterile PBS. All kids except 3 in each group were challenged twice at 75- and 275-day post-vaccination (DPV). Four goats each from three groups were sacrificed at 200-day post-challenge to evaluate carcass and histopathologically for vaccine and challenge response in kids of different groups. Samples (blood, serum and fecal) were screened for LTT, ELISA and shedding of bacilli and data on live animal traits, mortality and experimental sacrifice were compared. Average body weights gained by goats in three groups at different stages of trials (0, 1-75, 76-275, 276-425 DPV) showed marked improvements in performance of vaccinated groups over 'Sham-immunized' group. Effect of vaccines against challenge became visible in terms of body weights gained at 276-425 DPV ('Bison' group gained significantly higher body weights than 'Sham-immunized'). Mortality was significantly less in two vaccinated as compared to 'Sham-immunized'. Vaccinated groups also had significant stimulation and sero-conversion for cell mediated and humoral immune response, respectively as compared to 'Sham-immunized'. Results of post-challenged fecal culture showed significant reduction in shedding of MAP in both vaccinated groups than in 'Sham-immunized'. There was significant improvement in external and internal body traits and histological lesions in case of vaccinated than 'Sham-immunized' group.
机译：自1979年以来，位于马赫杜姆中央山羊研究所的山羊种中就普遍存在约翰德氏病（JD），最近在农户中也有相同频率的报道。尽管使用测试和屠宰方法控制这些猪群的JD超过25年，但JD的发病率并未降低。使用带有MAP同源菌株的攻毒研究，将包含禽分枝杆菌副物种（MAP）天然'Bison型'基因型的'本土疫苗'的功效与商业疫苗进行了比较。山羊孩子（85）被随机分为三组。给孩子皮下注射1ml疫苗，并用1ml无菌PBS进行假免疫。在接种后75天和275天（DPV），除每组3个孩子外，所有其他孩子均接受了两次挑战。攻击后200天分别处死三组的四只山羊，以评估car体，并在组织病理学上评估不同组别的孩子的疫苗和攻击反应。筛选样品（血液，血清和粪便）的LTT，ELISA和细菌脱落情况，并比较活体动物性状，死亡率和实验牺牲的数据。三组山羊在不同试验阶段（0、1-75、76-275、276-425 DPV）获得的平均体重显示，与“假免疫”组相比，接种组的性能明显改善。疫苗在276-425 DPV时获得的体重方面对抗挑战的效果变得显而易见（“野牛”组的体重明显高于“假免疫”组）。与“假免疫”相比，两次接种的死亡率显着降低。与“假免疫”相比，接种疫苗的组还分别具有明显的刺激和血清转化的细胞介导和体液免疫反应。挑战后的粪便培养结果表明，与“假免疫”组相比，两个疫苗接种组的MAP脱落均显着降低。与“假免疫”组相比，接种疫苗后的外部和内部身体特征以及组织学病变明显改善。

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