Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus

Reap EA; Morris J; Dryga SA; Maughan M; Talarico T; Esch RE; Negri S; Burnett B; Graham A; Olmsted RA; Chulay JD

首页> 外文期刊>Vaccine >Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus

【24h】

Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus

机译：用于巨细胞病毒的α病毒复制子颗粒疫苗的开发和临床前评估

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We used a replication-incompetent, single-cycle, alphavirus replicon vector system to produce virus-like replicon particles (VRP) expressing the extracellular domain of human cytomegalovirus (CMV) glycoprotein B or a pp65/IE1 fusion protein. Efficient production methods were scaled to produce pilot lots and clinical lots of each alphavirus replicon vaccine component. The vaccine induced high-titered antibody responses in mice and rabbits, as measured by ELISA and CMV neutralization assays, and robust T-cell responses in mice, as measured by IFN-gamma ELISPOT assay. A toxicity study in rabbits showed no adverse effects in any toxicology parameter. These studies support clinical testing of this novel CMV alphavirus replicon vaccine in humans.

机译：我们使用了无复制能力的单周期α病毒复制子载体系统，来生产表达人巨细胞病毒（CMV）糖蛋白B或pp65 / IE1融合蛋白胞外域的病毒样复制子颗粒（VRP）。缩放有效的生产方法以生产每种α病毒复制子疫苗成分的中试批次和临床批次。通过ELISA和CMV中和测定，该疫苗在小鼠和兔子中诱导出高滴度抗体应答，通过IFN-γELISPOT测定，在小鼠中诱导出强大的T细胞应答。对兔子的毒性研究表明，在任何毒理学参数方面均无不良影响。这些研究支持这种新型CMVα病毒复制子疫苗在人体中的临床测试。

著录项

来源
《Vaccine》 |2007年第42期|共9页
作者
Reap EA; Morris J; Dryga SA; Maughan M; Talarico T; Esch RE; Negri S; Burnett B; Graham A; Olmsted RA; Chulay JD;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类卫生标准、卫生检查、医药管理;
关键词

相似文献

外文文献
中文文献
专利

1. Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus [J] . Reap EA, Morris J, Dryga SA, Vaccine . 2007,第42期

机译：用于巨细胞病毒的α病毒复制子颗粒疫苗的开发和临床前评估
2. Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant [J] . Rivas-Aravena Andrea, Fuentes Yazmin, Cartagena Julio, Fish & Shellfish Immunology . 2015,第1期

机译：使用甲病毒复制子作为佐剂开发针对鲑鱼的纳米鲑鱼抗ISAV口服疫苗
3. Randomized, double-blind, Phase 1 trial of an alphavirus replicon vaccine for cytomegalovirus in CMV seronegative adult volunteers [J] . Bernstein David I., Reap Elizabeth A., Katen Kevin, Vaccine . 2010,第2期

机译：在巨细胞病毒血清阴性成人志愿者中针对巨细胞病毒的甲病毒复制子疫苗的随机，双盲，1期临床试验
4. Development of an alphavirus replicon particle-based vaccine against classical swine fever virus [C] . J. Dustin Loy, Mark Mogler, Jill Gander, Annual^Meeting of the American Association^of^Swine^Veterinarians. . 2013

机译：对古典猪瘟病毒的alphavirus复制子颗粒疫苗的研制
5. Characterization of alphavirus replicon particle based vaccines and adjuvants in a neonatal murine model. [D] . Khalil, Syed Muaz. 2014

机译：在新生鼠模型中表征基于α病毒复制子颗粒的疫苗和佐剂。
6. Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus [O] . Elizabeth A. Reap, John Morris, Sergey A. Dryga, -1

机译：用于巨细胞病毒的α病毒复制子颗粒疫苗的开发和临床前评估
7. Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus [O] . Elizabeth A. Reap, John Morris, Sergey A. Dryga, 2007

机译：甲状腺细胞病毒的alphavirus复制子颗粒疫苗的开发和临床前评价
8. Deinococcus Mn2+ -Peptide Complex: A Novel Approach to Alphavirus Vaccine Development. [R] . Gayen, M., Glass, P. J., Gupta, P., 2016

机译：Deinococcus mn2 + -peptide Complex：一种新的甲病毒疫苗开发方法。

Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus

摘要

著录项

相似文献

相关主题

期刊订阅