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首页> 外文期刊>Vaccine >The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant.
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The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant.

机译：来自共生的乳酸奈瑟氏球菌的PorB孔蛋白在小鼠中诱导对卵清蛋白的Th1和Th2免疫反应，是一种潜在的免疫佐剂。

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摘要

Porins from pathogenic Neisseriae are among several bacterial products with immune adjuvant activity. Neisseria meningitidis (Nme) PorB, has been shown to induce immune cells activation in a TLR2-dependent manner and acts as a vaccine immune adjuvant. The PorB porin from Neisseria lactamica (Nlac), a common nasopharyngeal commensal, shares significant structural and functional similarities with Nme PorB. In this work we ask whether the immune adjuvant ability of porins from pathogenic Neisserial strains is a characteristic shared with porins from non-pathogenic Neisserial species or whether it is unique for bacterial products derived from microorganisms capable of inducing inflammation and disease. We evaluated the potential immune adjuvant effect of Nlac PorB in mice using ovalbumin (OVA) as a prototype antigen. Immunization with Nlac PorB/OVA induced high OVA-specific IgG and IgM titers compared to OVA alone, similar to other adjuvants such as Nme PorB and alum. High titers of IgG1 and IgG2b were detected as well as production of IL-4, IL-10, IL-12 and INF-gamma in response to Nlac PorB, consistent with induction of both a Th1-type and a Th2-type immune response. OVA-specific proliferation was also determined in splenocytes from Nlac PorB/OVA-immunized mice. In addition, B cell activation in vitro and cytokine production in response to Nlac PorB was found to be mediated by TLR2, in a similar manner to Nme PorB.
机译：致病性奈瑟氏菌的孔蛋白属于几种具有免疫佐剂活性的细菌。脑膜炎奈瑟氏菌（Nme）PorB已显示以TLR2依赖性方式诱导免疫细胞活化，并充当疫苗免疫佐剂。奈瑟氏菌（Nlac）（一种常见的鼻咽粘膜）中的PorB孔蛋白与Nme PorB具有明显的结构和功能相似性。在这项工作中，我们询问致病性奈瑟氏菌菌株的孔蛋白的免疫佐剂功能是否与非致病性奈瑟氏菌种的孔蛋白具有相同的特征，或者它是否对于源自能够诱导炎症和疾病的微生物的细菌产品具有独特性。我们使用卵清蛋白（OVA）作为原型抗原评估了Nlac PorB在小鼠中的潜在免疫佐剂作用。与单独的OVA相比，与其他佐剂（如Nme PorB和明矾）相似，与单独的OVA相比，用Nlac PorB / OVA免疫诱导的OVA特异性IgG和IgM滴度高。检测到高滴度的IgG1和IgG2b以及响应Nlac PorB产生IL-4，IL-10，IL-12和INF-γ，与诱导Th1型和Th2型免疫反应一致。在Nlac PorB / OVA免疫小鼠的脾细胞中也确定了OVA特异性增殖。另外，发现与Nme PorB相似，TLR2介导了B细胞的体外活化和对Nlac PorB的反应中细胞因子的产生。

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