...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Protection of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum with a DNA vaccine containing the mutated zinc-metalloprotease gene
【24h】

Protection of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum with a DNA vaccine containing the mutated zinc-metalloprotease gene

机译：含有突变的锌金属蛋白酶基因的DNA疫苗对日本比目鱼（Paralichthys olivaceus）的抗鳗弧菌的保护

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vibrio anguillarum is one of the causative agents of vibriosis, a systemic disease of fish characterized by acute hemorrhagic septicemia. The extracellular zinc metalloprotease (EmpA) is a putative virulence factor involved in pathogenicity of V. anguillarum. Here we described the results of immunization against V. anguillarum with the plasmid expressing the mutated EmpA (m-EmpA7), which had no protelytic activity or cytotoxicity. In vitro protein expression of m-empA7 gene was determined by fluorescent microscopy and Western-blot after transfection of Chinese hamster ovary (CHO) and human embryonic kidney (HEK293T) cell lines. All three groups of fish immunized with a single intramuscular (i.m.) injection of different doses of the m-EmpA7 DNA vaccine showed significant serum antibody levels after vaccination, compared with the fish injected with the control eukaryotic expression vector pEGFP-N1 and PBS. In addition, fish receiving the DNA vaccine developed a protective response to a live V. anguillarum challenge 4 weeks post-inoculation, as demonstrated by increased survival of vaccinated fish over the control and by decreased histological alterations in vaccinated fish. Furthermore, humoral immune responses and protective effects were significantly increased at higher vaccine doses using a single intramuscularly injection route.
机译：鳗弧菌是弧菌病的病原体之一，弧菌病是一种以急性出血性败血病为特征的鱼类全身性疾病。细胞外锌金属蛋白酶（EmpA）是推测的毒力因子，与鳗弧菌的致病性有关。在这里，我们描述了用表达突变的EmpA（m-EmpA7）的质粒对鳗弧菌进行免疫的结果，该质粒没有蛋白水解活性或细胞毒性。转染中国仓鼠卵巢（CHO）和人胚肾（HEK293T）细胞系后，通过荧光显微镜和Western blot检测m-empA7基因的体外蛋白表达。与注射对照真核表达载体pEGFP-N1和PBS的鱼相比，接种不同剂量的m-EmpA7 DNA疫苗的单次肌内（i.m.）注射免疫的所有三组鱼均显示出明显的血清抗体水平。另外，接受DNA疫苗的鱼在接种后4周对活的鳗弧菌产生了保护性应答，这表现为接种鱼的存活率高于对照，并且接种鱼的组织学改变减少。此外，使用单个肌内注射途径，在较高疫苗剂量下，体液免疫反应和保护作用显着增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号