Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations.

Harmsen M. M.; Jansen J.; Westra D. F.; Coco-Martin J. M.

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Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations.

机译：在水和油乳剂配方中，通过表面增强的激光解吸电离-飞行时间质谱法表征口蹄疫病毒抗原。

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We have used a novel method, surface-enhanced laser desorption ionization-time of flight-mass spectrometry (SELDI-TOF-MS), to characterize foot-and-mouth disease virus (FMDV) vaccine antigens. Using specific capture with FMDV binding recombinant antibody fragments and tryptic digestion of FMDV antigens the spectral peaks representing the FMDV structural proteins VP1, VP2, VP3 and VP4 were identified. VP1 existed as 2 variants differing by 0.2 kDa and VP4 as 8 variants differing by 14-17 Da. Such heterogeneities have not been reported earlier. They could represent oxidation of VP4 and N-glycation of VP1. We also detected FMDV proteolysis upon incubation at elevated temperatures and impurities in FMDV antigen preparations. Finally, we could also characterize FMDV antigen present in emulsions with oil adjuvant by SELDI-TOF-MS. Such FMDV antigen retained the VP4 protein which is known to be specifically present in intact (146S) FMDV particles but absent from specific (12S) degradation products. This indicates that virions do not dissociate upon emulsification.

机译：我们使用了一种新颖的方法，即飞行质谱仪（SELDI-TOF-MS）的表面增强激光解吸电离时间，以表征口蹄疫病毒（FMDV）疫苗抗原。使用结合FMDV的重组抗体片段进行特异性捕获和FMDV抗原的胰蛋白酶消化，鉴定出代表FMDV结构蛋白VP1，VP2，VP3和VP4的光谱峰。 VP1以2个变异形式存在，相差0.2 kDa，VP4以8个变异形式存在，相差14-17 Da。这种异质性尚未在早期报道。它们可能代表VP4的氧化和VP1的N-糖基化。我们还通过在高温下孵育和FMDV抗原制剂中的杂质检测到FMDV蛋白水解。最后，我们还可以通过SELDI-TOF-MS表征含油佐剂的乳剂中存在的FMDV抗原。此类FMDV抗原保留了VP4蛋白，已知该蛋白特别存在于完整的（146S）FMDV颗粒中，但不存在于特定的（12S）降解产物中。这表明病毒粒子在乳化时不会解离。

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来源
《Vaccine》 |2010年第19期|共8页
作者
Harmsen M. M.; Jansen J.; Westra D. F.; Coco-Martin J. M.;
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原文格式 PDF
正文语种 eng
中图分类医学免疫学;
关键词
analytical methods; animal diseases; antibodies; antigens; cell cultures; characterization; detection; emulsions; foot and mouth disease; formulations; in vitro; MALDI-TOF; mass spectrometry; proteolysis; vaccines; viral diseases; Foot-and-mouth disease virus; Netherlands;

机译：分析方法;动物疾病;抗体;抗原;细胞培养;鉴定;检测;乳剂;口蹄疫;制剂;体外;MALDI-TOF;质谱法;蛋白水解;疫苗;病毒性疾病;口蹄疫病毒;荷兰;

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1. Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations. [J] . Harmsen M. M., Jansen J., Westra D. F., Vaccine . 2010,第19期

机译：在水和油乳剂配方中，通过表面增强的激光解吸电离-飞行时间质谱法表征口蹄疫病毒抗原。
2. Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry for detection of protein expression profiles in the hippocampus following closed brain injury [J] . Qingming Shu, Zhiqiang Li, Shuwang Yang, 中国神经再生研究：英文版 . 2010,第023期

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3. Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS): A new proteomic urinary test for patients with urolithiasis. [J] . Cadieux PA, Beiko DT, Watterson JD, Journal of clinical laboratory analysis. . 2004,第3期

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4. Optimization of Sample Preparation for Detection of Common Food-borne Pathogens by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [C] . Lay Hoon Seah, Tze Horng Liew, Aldrich Leow, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：通过基质辅助激光解吸电离 - 飞行质谱法检测常见食品传播病原体的样品制剂的优化
5. Nonpolar matrices for matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. [D] . Robins, Chad LaJuan. 2005

机译：非极性矩阵用于基质辅助激光解吸/电离飞行时间质谱。
6. Weak cation exchange magnetic beads coupled with matrix-assisted laser desorption ionization-time of flight-mass spectrometry in screening serum protein markers in osteopenia [O] . Wei-Tao He, Bo-Cheng Liang, Zhen-Yu Shi, -1

机译：弱阳离子交换磁珠结合基质辅助激光解吸电离-飞行时间质谱法检测骨质减少的血清蛋白标志物
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机译：弱阳离子交换磁珠结合基质辅助激光解吸电离-飞行时间质谱法检测骨质减少的血清蛋白标志物

Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations.

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