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首页> 外文期刊>Vaccine >Oral immunization with a novel lipid-based adjuvant protects against genital Chlamydia infection.
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Oral immunization with a novel lipid-based adjuvant protects against genital Chlamydia infection.

机译：新型基于脂质的佐剂口服免疫可预防生殖道衣原体感染。

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Oral immunization is attractive as a delivery route because it is needle-free and useful for rapid mass vaccination programs to target pandemics or bioterrorism. This potential has not been realized for human vaccination, due to the requirement of large antigen doses and toxic (to humans) adjuvants to overcome the induction of oral tolerance and potential degradation of antigens in the stomach. To date, only oral vaccines based on live attenuated organisms have been approved for human use. In this study we describe the use of a lipid-based delivery system/adjuvant, Lipid C, for oral immunization to protect mice against genital tract chlamydial infection. Lipid C is formulated from food-grade purified and fractionated triglycerides. Bacterial shedding following vaginal challenge with Chlamydia muridarum was reduced by 50% in female mice orally immunized with the chlamydial major outer membrane protein (MOMP) formulated in Lipid C, protection equivalent to that seen in animals immunized with MOMP admixed with both cholera toxin (CT) and CpG oligodeoxynucleotides (CpG-ODN). Protection was further enhanced when MOMP, CT and CpG were all combined in the Lipid C matrix. Protection correlated with production of gamma interferon (IFN) by splenic T cells, a serum MOMP-specific IgG response and low but detectable levels of MOMP-specific IgA in vaginal lavage.
机译：口服免疫作为一种传递途径是有吸引力的，因为它无针且可用于针对大规模流行病或生物恐怖主义的快速大规模疫苗接种计划。由于需要大剂量的抗原和对人类有毒的佐剂，以克服口服耐受性的诱导和抗原在胃中的潜在降解，因此尚未实现人类疫苗接种的潜力。迄今为止，仅基于减毒活生物体的口服疫苗已被批准用于人类。在这项研究中，我们描述了基于脂质的递送系统/佐剂脂质C的口服免疫，以保护小鼠免受生殖道衣原体感染。脂质C由食品级纯化和分馏的甘油三酸酯配制而成。经脂质脂C配制的衣原体主要外膜蛋白（MOMP）口服免疫的雌性小鼠经阴道衣原体感染后，细菌脱落减少了50％，其保护作用与经MOMP免疫的动物相同与霍乱毒素（CT）和CpG寡脱氧核苷酸（CpG-ODN）混合。当MOMP，CT和CpG都结合在脂质C基质中时，保护作用进一步增强。保护作用与脾T细胞产生的γ干扰素（IFN），血清MOMP特异性IgG反应以及阴道灌洗液中MOMP特异性IgA水平低但可检测到的水平相关。

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