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首页> 外文期刊>Vaccine >Adjuvant effects of chicken interleukin-18 in avian Newcastle disease vaccine.
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Adjuvant effects of chicken interleukin-18 in avian Newcastle disease vaccine.

机译：鸡白细胞介素18在禽新城疫疫苗中的佐剂作用。

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摘要

Interleukin-18 (IL-18) can induce interferon- gamma (IFN- gamma ) production and promote Th1 immunity, and hence, it modulates immune functions. In the present study, the in vitro and in vivo immunomodulatory activities of full length or mature chicken IL-18 expressed in a prokaryotic expression system (pCHIL18-F and pCHIL18-M, respectively) and chicken IL-18 expressed in a eukaryotic expression system (euCHIL18) were examined. Results showed that pCHIL18-F, pCHIL18-M and euCHIL18 significantly enhanced IFN- gamma mRNA expression in chicken splenocytes, which successfully increased IFN- gamma -induced nitric oxide (NO) synthesis by macrophages. Vaccination with cell-cultured Newcastle disease vaccine (NDTC) co-administrated with pCHIL18-F, pCHIL18-M or euCHIL18 resulted in significant increments of hemagglutination inhibition (HI) titers, cell proliferation of peripheral blood mononuclear cells (PBMC), and ratios of CD8+ to CD4+ in chickens compared with inoculation of PBS or NDTC alone. Thus, full length and mature chicken IL-18 expressed using a prokaryotic system and using a eukaryotic system showed equivalent in vitro and in vivo biological activities, and all forms effectively enhanced cell-mediated and humoral immunity, suggesting possible future use as a potential adjuvant in chicken NDTC vaccine production.
机译：白介素-18（IL-18）可以诱导干扰素-γ（IFN-γ）的产生并促进Th1免疫力，因此可以调节免疫功能。在本研究中，全长或成熟鸡IL-18的体外和体内免疫调节活性在原核表达系统（pCHIL18-F和pCHIL18-M）中表达分别检测了真核表达系统（euCHIL18）中表达的鸡IL-18。结果显示，pCHIL18-F，pCHIL18-M和euCHIL18显着增强了鸡脾细胞中IFN-γ的表达，从而成功地增强了巨噬细胞对IFN-γ诱导的一氧化氮（NO）合成的作用。与pCHIL18-F，pCHIL18-M或euCHIL18共同施用的细胞培养新城疫疫苗（NDTC）的疫苗接种可导致血凝抑制（HI）滴度，外周血单核细胞（PBMC）的细胞增殖以及与单独接种PBS或NDTC相比，鸡的CD8 + 至CD4 + 。因此，使用原核系统和真核系统表达的全长和成熟的鸡IL-18表现出等效的体外和体内生物活性，并且所有形式均能有效增强细胞介导的和体液免疫，表明将来有可能用作鸡NDTC疫苗生产中的潜在佐剂。

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