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首页> 外文期刊>Vaccine >Induction of mucosal and systemic immune responses following oral immunization of mice with Lactococcus lactis expressing human papillomavirus type 16 L1
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Induction of mucosal and systemic immune responses following oral immunization of mice with Lactococcus lactis expressing human papillomavirus type 16 L1

机译：用表达人乳头瘤病毒16型L1的乳酸乳球菌口服免疫小鼠后诱导粘膜和全身免疫反应

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摘要

Human papillomavirus type 16 L1 (HPV16 L1) has shown considerable promise as a parenteral vaccine for prevention of cervical cancers. Here, we report the possibility of oral vaccination for HPV16 L1 using Lactococcus lactis (L. lactis) as a live vector. L. lactis MG1363 was transformed with two types of HPV16 L1-encoding plasmids for intracellular expression or secretion. L. lactis transformed with HPV16 L1-encoding plasmids retained biochemical lactic acid production capability. The mucosal and systemic immune responses were affected by the cellular location of expressed HPV16 L1 proteins in L. lactis. Serum IgG responses were induced after oral immunizations of L. lactis secreting HPV16 L1. Vaginal IgA immune responses were observed following oral immunization with L. lactis expressing HPV16 L1 in an intracellular form, but not with L. lactis secreting HPV16 L1. Furthermore, induction of HPV16 L1-specific mucosal immune responses was affected by immunization frequency. Six immunizations over 5 weeks were required to induce vaginal immune responses. The levels of HPV16 L1-specific vaginal IgA were maintained until 12 weeks after the first vaccination. These results suggest the feasibility of L. lactis as an oral vaccine vehicle of HPV16 L1 and demonstrate the importance of cellular loci of expressed antigen for induction of vaginal and systemic immune responses.
机译：人类乳头瘤病毒16 L1型（HPV16 L1）作为预防宫颈癌的肠胃外疫苗已显示出巨大的前景。在这里，我们报告了使用乳酸乳球菌（乳酸乳球菌）作为活载体口服HPV16 L1疫苗的可能性。用两种类型的HPV16 L1编码质粒转化乳酸乳球菌MG1363，以进行细胞内表达或分泌。用HPV16 L1编码质粒转化的乳酸乳杆菌保留了生化乳酸的生产能力。黏膜和全身免疫反应受乳酸乳球菌中表达的HPV16 L1蛋白在细胞中的位置影响。口服免疫分泌HPV16 L1的乳酸乳球菌后，可诱导血清IgG反应。口服IgA乳酸菌以细胞内表达HPV16 L1的乳酸乳球菌免疫接种后，观察到阴道IgA免疫反应，而分泌HPV16 L1的乳酸乳球菌则口服免疫。此外，HPV16 L1特异性粘膜免疫应答的诱导受到免疫频率的影响。在5周内需要进行六次免疫接种才能诱发阴道免疫反应。 HPV16 L1特异性阴道IgA的水平一直维持到第一次接种后12周。这些结果表明乳酸乳球菌作为HPV16 L1的口服疫苗载体的可行性，并证明了表达抗原的细胞基因座对于诱导阴道和全身免疫应答的重要性。

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