...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry.
【24h】

Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry.

机译：具有不同大小的非结构（NS）基因的流感病毒变异体的表征及其作为活禽流感疫苗的潜力。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

From a stock of A/turkey/Oregon/71-delNS1 (H7N3) virus, which has a 10 nucleotide deletion in the coding region of the NS1 gene, we found that several variants with different sizes of NS genes could be produced by passaging the virus in 10- and 14-day-old embryonating chicken eggs (ECE), but not in 7-day-old ECE or Vero cells. We were able to rescue the reassortant virus that has different sizes of the NS genes and confirmed that those NS genes are genetically stable. By conducting in vivo studies in 2-week-old chickens, we found two plaque purified variants (D-del pc3 and pc4) which can be used as a potential live-attenuated vaccine. The variants were highly attenuated in chickens and did not transmit the virus from infected chickens to uninoculated cage mates. At the same time, the variants induced relatively high antibody titers which conferred good protection against a high dose heterologous virus challenge. Our study indicates that naturally selected NS1 deletion variants might be useful in the development of live-attenuated influenza vaccines in poultry. Furthermore, deletion in the NS1 protein can be potentially useful as a negative marker for a differentiating infected from vaccinated animals (DIVA) approach.
机译：从库存的A /火鸡/俄勒冈/ 71-delNS1（H7N3）病毒中，NS1基因的编码区缺失了10个核苷酸，我们发现，通过传代这种病毒在10天和14天大的雏鸡卵（ECE）中出现，但在7天大的ECE或Vero细胞中没有。我们能够挽救具有不同NS基因大小的重配病毒，并确认这些NS基因具有遗传稳定性。通过对两周大的鸡进行体内研究，我们发现了两个噬菌斑纯化的变体（D-del pc3和pc4），可用作潜在的减毒活疫苗。这些变种在鸡中高度减毒，并且不会将病毒从受感染的鸡传播到未接种的笼伴侣。同时，这些变体诱导了相对较高的抗体滴度，从而针对高剂量异源病毒攻击提供了良好的保护。我们的研究表明，自然选择的NS1缺失变体可能对家禽减毒活疫苗的开发有用。此外，NS1蛋白中的缺失可能可作为阴性标记，用于区分感染的动物和接种的动物（DIVA）方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号