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首页> 外文期刊>Vaccine >Characterization of the interactions of the pneumolysoid, [Greek capital Delta]6 PLY, with human neutrophils in vitro.
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Characterization of the interactions of the pneumolysoid, [Greek capital Delta]6 PLY, with human neutrophils in vitro.

机译：体外表征气溶体，希腊资本Δ6PLY与人中性粒细胞的相互作用。

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摘要

The pneumolysin toxoid, [Greek capital Delta]6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P<0.05) release of MMP-9 and generation of LTB(4). However, treatment of the cells with [Greek capital Delta]6 PLY at concentrations of up to 1000 ng/ml had only trivial effects on Ca(2+) influx and no effects on either release of MMP-9 or LTB(4) production. The observed absence of pro-inflammatory interactions of [Greek capital Delta]6 PLY with neutrophils is clearly an important property of this pneumococcal protein vaccine candidate. Copyright Copyright 2011 Elsevier Ltd. All rights reserved.
机译：肺炎球菌溶血素类毒素，希腊首都Delta 6 PLY，是肺炎球菌蛋白疫苗的原型候选物。然而，其与人嗜中性粒细胞的潜在有害残留促炎相互作用尚不清楚。在当前的研究中，类毒素（8-1000 ng / ml）与野生型肺炎球菌溶血素（WT / PLY，8 ng / ml）对中性粒细胞胞质Ca（2+）通量，白三烯生成的影响进行了比较B（4）（LTB（4））和基质金属蛋白酶9（MMP-9）的释放，分别使用光谱荧光和ELISA程序（LTB（4）和MMP-9）。中性粒细胞暴露于WT / PLY导致Ca（2+）流入和MMP-9的显着（P <0.05）释放以及LTB（4）的产生。但是，用浓度高达1000 ng / ml的希腊资本Δ6PLY处理细胞对Ca（2+）流入仅具有微不足道的影响，而对MMP-9或LTB（4）生产的释放均无影响。 。所观察到的缺乏希腊资本Δ6PLY与中性粒细胞的促炎性相互作用显然是该肺炎球菌蛋白疫苗候选物的重要性质。版权版权所有2011 ElsevierLtd。保留所有权利。

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