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首页> 外文期刊>Vaccine >Different patterns of expansion, contraction and memory differentiation of HIV-1 Gag-specific CD8 T cells elicited by adenovirus type 5 and modified vaccinia Ankara vaccines.
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Different patterns of expansion, contraction and memory differentiation of HIV-1 Gag-specific CD8 T cells elicited by adenovirus type 5 and modified vaccinia Ankara vaccines.

机译：由5型腺病毒和修饰的牛痘安卡拉疫苗引发的HIV-1 Gag特异性CD8 T细胞的不同扩增，收缩和记忆分化模式。

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摘要

The magnitude and functional quality of antiviral CD8 T cell responses are critical for the efficacy of T cell based vaccines. Here, we investigate the influence of two popular viral vectors, adenovirus type 5 (Ad5) and modified vaccinia Ankara (MVA), on expansion, contraction and memory differentiation of HIV-1 Gag insert-specific CD8 T cell responses following immunization and show different patterns for the two recombinant viral vectors. The Ad5 vector primed 6-fold higher levels of insert-specific CD8 effector T cells than the MVA vector. The Ad5-primed effector cells also underwent less contraction (<2-fold) than the MVA-primed cells (>5-fold). The Ad5-primed memory cells were predominantly CD62L negative (effector memory) whereas the MVA-primed memory cells were predominantly CD62L positive (central memory). Consistent with their memory phenotype, MVA-primed CD8 T cells underwent higher fold expansion than Ad5-primed CD8 T cells following a homologous or heterologous boost. Impressively, the Ad5 boost changed the quality of MVA-primed memory response such that they undergo less contraction with effector memory phenotype. However, the MVA boost did not influence the contraction and memory phenotype of Ad5-primed response. In conclusion, our results demonstrate that vaccine vector strongly influences the expansion, contraction and the functional quality of insert-specific CD8 T cell responses and have implications for vaccine development against infectious diseases.
机译：抗病毒CD8 T细胞反应的强度和功能质量对于基于T细胞的疫苗的功效至关重要。在这里，我们调查两种流行的病毒载体，腺病毒5型（Ad5）和修饰的痘苗安卡拉（MVA），对免疫后HIV-1 Gag插入特异性CD8 T细胞反应的扩增，收缩和记忆分化的影响，并显示出不同的两个重组病毒载体的模式。 Ad5载体引发的插入特异性CD8效应T细胞的水平是MVA载体的6倍。与MVA引发的细胞（> 5倍）相比，Ad5引发的效应细胞也经历了更少的收缩（<2倍）。 Ad5启动的记忆细胞主要是CD62L阴性（效应记忆），而MVA启动的记忆细胞主要是CD62L阳性（中央记忆）。与它们的记忆表型一致，在同源或异源加强后，由MVA启动的CD8 T细胞比由Ad5启动的CD8 T细胞经历更高的倍数扩展。令人印象深刻的是，Ad5增强改变了MVA启动的记忆反应的质量，从而使它们在效应记忆表型的作用下减少了收缩。但是，MVA增强并不影响Ad5启动反应的收缩和记忆表型。总之，我们的结果表明，疫苗载体强烈影响插入特异性CD8 T细胞反应的扩增，收缩和功能质量，并且对开发针对传染病的疫苗有影响。

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