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首页> 外文期刊>Vaccine >A DNA vaccine candidate encoding the structural prM/E proteins elicits a strong immune response and protects mice against dengue-4 virus infection.
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A DNA vaccine candidate encoding the structural prM/E proteins elicits a strong immune response and protects mice against dengue-4 virus infection.

机译：编码结构性prM / E蛋白的DNA疫苗候选物引起强烈的免疫反应，并保护小鼠免受登革4病毒感染。

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A DNA vaccine expressing dengue-4 virus premembrane (prM) and envelope (E) genes was produced by inserting these genes into a mammalian expression plasmid (pCI). Following a thorough screening, including confirmation of protein expression in vitro, a recombinant clone expressing these genes was selected and used to immunize BALB/c mice. After 3 immunizations all the animals produced detectable levels of neutralizing antibodies against dengue-4 virus. The cytokines levels and T cell proliferation, detected ex vivo from the spleen of the immunized mice, showed that our construction induced substantial immune stimulation after three doses. Even though the antibody levels, induced by our DNA vaccine, were lower than those obtained in mice immunized with dengue-4 virus the levels of protection were high with this vaccine. This observation is further supported by the fact that 80% of the vaccine immunized group was protected against lethal challenge. In conclusion, we developed a DNA vaccine employing the genes of the prM and E proteins from dengue-4 virus that protects mice against this virus.Digital Object Identifier http://dx.doi.org/10.1016/j.vaccine.2010.10.078
机译：通过将登革4病毒前膜（prM）和包膜（E）基因插入哺乳动物表达质粒（pCI）中来生产表达DNA疫苗。经过全面的筛选，包括体外蛋白质表达的确认，选择了表达这些基因的重组克隆并用于免疫BALB / c小鼠。免疫3次后，所有动物均产生可检测水平的抗登革4病毒中和抗体。从被免疫小鼠的脾脏中离体检测到的细胞因子水平和T细胞增殖表明，我们的构建物在三剂后诱导了实质性的免疫刺激。尽管我们的DNA疫苗诱导的抗体水平低于用登革4病毒免疫的小鼠中获得的抗体水平，但该疫苗的保护水平却很高。疫苗免疫组中80％免受致命性攻击的事实进一步支持了这一观察结果。最后，我们开发了一种DNA疫苗，该疫苗使用了来自登革4病毒的prM和E蛋白的基因来保护小鼠免受该病毒的侵害。数字对象标识符http://dx.doi.org/10.1016/j.vaccine.2010.10。 078

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