...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies
【24h】

Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies

机译：口服免疫研究中使用的植物来源的乙型肝炎表面抗原的结构表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Several subunit vaccine antigens have been successfully expressed in plants and recently the hepatitis B surface antigen (HBsAg), expressed in potatoes, was shown to be orally immunogenic in animal studies. However, to date, a detailed analysis of the plant-derived antigen is lacking. Herein, we comprehensively characterize the structure and post-translational processing of HBsAg from potato tuber and two plant cell suspension cultures. The HBsAg was found to accumulate intracellularly as tubular structures, with a complex size distribution, differing substantially from the virus-like particle (VLP) preparations of the current commercial vaccines. Extensive disulfide-bond cross-linking, which is important for immunogenicity, was evident and 21-37% of total HBsAg protein displayed epitopes which correlate with vaccine potency. The significance of these results with regard to the production of cost-effective orally delivered vaccines is discussed.
机译：几种亚单位疫苗抗原已在植物中成功表达，最近在马铃薯中表达的乙型肝炎表面抗原（HBsAg）在动物研究中显示出口服免疫原性。但是，迄今为止，缺乏对植物来源的抗原的详细分析。本文中，我们全面表征了马铃薯块茎和两种植物细胞悬浮培养物中HBsAg的结构和翻译后加工过程。已发现HBsAg以管状结构形式在细胞内积累，具有复杂的大小分布，与目前商业疫苗的病毒样颗粒（VLP）制剂大不相同。很明显，广泛的二硫键交联对免疫原性很重要，总HBsAg蛋白的21-37％显示出与疫苗效力相关的表位。讨论了这些结果对生产具有成本效益的口服疫苗的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号