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首页> 外文期刊>Vaccine >A prime-boost immunization strategy with DNA and recombinant baculovirus-expressed protein enhances protective immunogenicity of glycoprotein D of equine herpesvirus 1 in naive and infection-primed mice.
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A prime-boost immunization strategy with DNA and recombinant baculovirus-expressed protein enhances protective immunogenicity of glycoprotein D of equine herpesvirus 1 in naive and infection-primed mice.

机译：DNA和重组杆状病毒表达的蛋白的初免-加强免疫策略增强了天真和感染后小鼠的马疱疹病毒1糖蛋白D的保护性免疫原性。

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摘要

The immunogenicity and protective efficacy afforded by intramuscular inoculation of plasmid DNA encoding equine herpesvirus 1 (EHV-1) glycoprotein D (gD) followed by EHV-1 gD expressed by a recombinant baculovirus was assessed in a murine model of EHV-1 respiratory infection. Compared with mice inoculated with DNA or protein only, mice inoculated with the combination of gD DNA and protein had enhanced ELISA and neutralizing antibody titres to EHV-1 and had accelerated clearance of virus from lungsfollowing challenge infection. The enhanced protective effects of this consecutive immunization were also evident in mice which had a previous infection with EHV-1 and had pre-existing antibodies. The T-helper 1 (Th 1) type of immune response induced byEHV-1 gD DNA was maintained after the protein boost, despite the gD protein alone appearing to direct a Th2 response. It is concluded that the enhanced immunogenicity of EHV-1 gD in the mouse model using consecutive immunization with DNA and protein indicates that this antigen and the prime-boost strategy may be useful in generating protective immunity against EHV-1 in horses.
机译：在EHV-1呼吸道感染的小鼠模型中评估了肌肉注射编码马疱疹病毒1（EHV-1）糖蛋白D（gD）的质粒DNA，然后重组重组杆状病毒表达的EHV-1 gD所提供的免疫原性和保护效力。与仅接种DNA或蛋白质的小鼠相比，接种gD DNA和蛋白质的小鼠具有增强的ELISA和中和针对EHV-1的抗体滴度，并在挑战感染后加速了从肺中清除病毒。这种连续免疫的增强保护作用在先前曾感染过EHV-1并已预先存在抗体的小鼠中也很明显。尽管单独的gD蛋白似乎指导Th2反应，但在蛋白加强后，由EHV-1 gD DNA诱导的T-helper 1（Th 1）类型的免疫反应仍得以维持。结论是，在小鼠模型中使用DNA和蛋白质连续免疫增强了EHV-1 gD的免疫原性，表明该抗原和初免-加强免疫策略可能对产生针对马的EHV-1的保护性免疫有用。

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