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Enhanced cellular immune responses to SIV Gag by immunization with influenza and vaccinia virus recombinants

机译：通过流感和牛痘病毒重组体免疫增强对SIV Gag的细胞免疫反应

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An effective vaccination strategy against human immunodeficiency virus type 1 (HIV-1) should include the induction of potent cellular immune responses against conserved HIV-1 antigens. We have generated five replication competent recombinant influenza viruses (rFlu/SIV Gag nos. 1-5) expressing different portions of Gag of simian immunodeficiency virus (SIV). Single intranasal immunizations in mice with each rFlu/SIV Gag viruses resulted in different degrees of protection against a challenge with recombinant vaccinia virus expressing SIV Gag. Immunized BALB/c mice had detectable CD8+ T cell responses specific for Gag peptide 185-199 when mice were vaccinated with rFlu/SIV Gag no. 3 virus, and for Gag peptides 281-295 and 285-299 when vaccinated with rFlu/SIV Gag no. 4 virus. Cellular immune responses against SIV Gag were further enhanced by a booster with a recombinant vaccinia virus expressing SIV Gag in both the spleen and local lymph node tissues, resulting in the induction of robust Gag-specific CD8+ T cell responses at both systemic and mucosal levels. We suggest that a prime-boost immunization regimen using recombinant influenza and vaccinia viruses expressing HIV Gag might represent an effective means to induce potent HIV-specific, protective CD8+ T cell responses.
机译：针对人类1型免疫缺陷病毒（HIV-1）的有效疫苗接种策略应包括针对保守的HIV-1抗原诱导有效的细胞免疫应答。我们已经产生了五种表达复制能力的重组流感病毒（rFlu / SIV Gag第1-5号），它们表达猿猴免疫缺陷病毒（SIV）Gag的不同部分。用每种rFlu / SIV Gag病毒在小鼠中进行单次鼻内免疫可对表达SIV Gag的重组牛痘病毒的攻击产生不同程度的保护。当小鼠用rFlu / SIV Gag no.1接种时，免疫的BALB / c小鼠具有对Gag肽185-199有特异性的可检测到的CD8 + T细胞应答。 3种病毒，以及用rFlu / SIV Gag疫苗接种的Gag肽281-295和285-299。 4病毒。通过在脾脏和局部淋巴结组织中均表达SIV Gag的重组牛痘病毒加强免疫，可以进一步增强针对SIV Gag的细胞免疫应答，从而在全身和粘膜水平上均诱导出强大的Gag特异性CD8 + T细胞应答。我们建议使用表达HIV Gag的重组流感和牛痘病毒的初免加强免疫方案可能是诱导有效的HIV特异性，保护性CD8 + T细胞应答的有效手段。

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