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首页> 外文期刊>Vaccine >Vaccination of Swiss albino mice against experimental visceral leishmaniasis with the FML antigen of Leishmania donovani
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Vaccination of Swiss albino mice against experimental visceral leishmaniasis with the FML antigen of Leishmania donovani

机译：用白喉利什曼原虫的FML抗原对瑞士白化病小鼠进行实验性内脏利什曼病的疫苗接种

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摘要

The FML antigen (a glycoprotein-enriched ligand containing fucose, mannose, glucose and galactose) of Leishmania donovani, in combination with saponin, aluminium hydroxide (Al(OH)3) and Freund's incomplete adjuvant (FIA), was used in vaccines testedin an outbred murine model of visceral leishmaniasis, via intraperitoneal (ip) and subcutaneous (sc) routes. The humoral response was significantly higher in mice treated with FML + saponin or FML + Al(OH)3 compared with in controls, both before and after infection. Mice immunized by the ip route developed higher antibody titres. A significant and specific reduction of parasite load in relation to saline (85%, P<0.01) and saponin (P<0.025) controls was seen in mice treated with FML + saponin by ip. Coincidentally with this, an increase in IgG2a antibodies was detected only in animals treated with FML + saponin ip. A reduction of 88% in parasite load was achieved by the combination of FML + Al(OH)3 (sc), but Al(OH)3 treatment by itself accounted for 68% of this protection. Vaccination with FML + saponin ip was superior to the other treatments and showed no toxic effect due to saponin.
机译：Leishmania donovani的FML抗原（富含果糖，甘露糖，葡萄糖和半乳糖的富糖蛋白配体）与皂苷，氢氧化铝（Al（OH）3）和弗氏不完全佐剂（FIA）结合使用，用于疫苗内脏利什曼病的杂种鼠模型，通过腹膜内（ip）和皮下（sc）途径。在感染前后，用FML +皂苷或FML + Al（OH）3处理的小鼠的体液反应均显着高于对照组。经腹膜内途径免疫的小鼠产生更高的抗体滴度。在腹腔注射FML +皂苷处理的小鼠中，与盐水（85％，P <0.01）和皂苷（P <0.025）对照相比，寄生虫负荷显着且特异性降低。与此巧合的是，仅在FML +皂苷ip处理的动物中检测到IgG2a抗体增加。 FML + Al（OH）3（sc）的结合可降低88％的寄生虫负荷，但Al（OH）3处理本身占了这种保护的68％。 FML +皂苷腹膜内注射疫苗优于其他治疗方法，并且由于皂苷没有显示毒性作用。

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