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Size determination of bacterial capsular oligosaccharides used to prepareconjugate vaccines

机译：用于制备偶联疫苗的细菌荚膜寡糖的大小确定

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We recently described the use of ion exchange chromatography for analysis and the industrial scale preparation of pools of oligsaccharides of intermediate chain length from polysaccharides of Haemophilus influenzae of type b (Hib) and Neisseria meningitidis groups A and C. These negatively charged "sized" oligosaccharides are activated and conjugated to the carrier protein (CRM197) to prepare the corresponding glycoconjugate vaccines. Characterization and accurate determination of the degree of polymerization (DP) of the pool of oligosaccharides is essential for the consistent production of these conjugate vaccines. This paper describes the colorimetric assays used for determination of the average DP of the Hib and meningococcal oligosaccharides, and the qualification of these assays achieved by size characterization of the respective oligosaccharides by use of physicochemical methods, including liquid chromatography. mass spectrometry (ionspray) and NMR spectroscopy. (C) 1999 Elsevier Science Ltd. All rights reserved. [References: 27]
机译：我们最近描述了使用离子交换色谱从b型流感嗜血杆菌（Hib）以及脑膜炎奈瑟氏球菌A和C组的多糖中链长度的寡糖的分析和工业规模制备中的应用。将其活化并偶联至载体蛋白（CRM197）以制备相应的糖缀合物疫苗。寡糖池的聚合度（DP）的表征和准确测定对于稳定生产这些结合疫苗至关重要。本文介绍了用于测定Hib和脑膜炎球菌寡糖平均DP的比色测定法，以及通过使用物理化学方法（包括液相色谱法）对各个寡糖进行大小表征获得的这些测定法的资格。质谱（离子喷​​雾）和NMR光谱。 （C）1999 Elsevier ScienceLtd。保留所有权利。 [参考：27]

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