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首页> 外文期刊>Vaccine >Ginseng stem-leaf saponins (GSLS) and mineral oil act synergistically to enhance the immune responses to vaccination against foot-and-mouth disease in mice
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Ginseng stem-leaf saponins (GSLS) and mineral oil act synergistically to enhance the immune responses to vaccination against foot-and-mouth disease in mice

机译：人参茎叶皂苷（GSLS）和矿物油协同作用，增强小鼠预防口蹄疫疫苗的免疫反应

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摘要

Saponins extracted from ginseng stems and leaves (GSLS) as well as the synergistic effect between GSLS and oil emulsion were investigated for their adjuvant effects on the immune responses of mice to vaccination against foot-and-mouth disease virus (FMDV) serotype Asia 1. In experiment A, ICR mice were subcutaneously immunized twice with FMDV antigen with or without GSLS (0, 1, 5, 10 and 20mug) at 3 week intervals. Highest FMDV-specific IgG level was observed 2 weeks after the boosting in mice immunized with FMDV antigen plus 10mug of GSLS. In experiment B, mice were subcutaneously injected with FMDV antigen with or without GSLS (10mug), or in oil emulsion with or without GSLS (10mug) on days 1 and 21. Results indicated that when co-administered with a mixture of oil and GSLS, FMDV antigen induced significantly higher IgG titer and IgG1, IgG2a, IgG2b and IgG3 responses, production of IFN-gamma (Th1 cytokine) and IL-5 (Th2 cytokine) by splenocytes, as well as T and B lymphocyte proliferation in response to Con A and LPS than when FMDV antigen was used alone or mixed with either GSLS or oil. This suggests that GSLS and oil adjuvant synergistically promote both Th1 and Th2 immune responses. As protection against FMDV requires both cellular and humoral immune responses, the combined effects of GSLS and oil deserve further study in other animals such as cattle and pigs in order to induce effective immunity against FMDV infection.
机译：研究了从人参茎叶（GSLS）中提取的皂苷以及GSLS与油乳剂之间的协同作用，以其对小鼠针对口蹄疫病毒（FMDV）亚洲1型血清型的免疫反应的佐剂作用。在实验A中，以FMDV抗原在有或没有GSLS（0、1、5、10和20杯）的情况下以3周的间隔皮下免疫ICR小鼠两次。在用FMDV抗原加10杯GSLS免疫的小鼠加强免疫后2周，观察到最高的FMDV特异性IgG水平。在实验B中，在第1天和第21天对小鼠皮下注射有或没有GSLS的FMDV抗原（10杯），或在有或没有GSLS的油乳剂中（10马克）皮下注射。 ，FMDV抗原诱导明显更高的IgG滴度和IgG1，IgG2a，IgG2b和IgG3应答，脾细胞产生IFN-γ（Th1细胞因子）和IL-5（Th2细胞因子），以及响应Con的T和B淋巴细胞增殖与单独使用FMDV抗原或与GSLS或油混合使用A和LPS相比。这表明GSLS和油佐剂协同促进Th1和Th2免疫反应。由于针对FMDV的保护需要细胞和体液免疫应答，因此GSLS和油的联合作用值得在其他动物（如牛和猪）中进行进一步研究，以诱导针对FMDV感染的有效免疫力。

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