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首页> 外文期刊>Vaccine >Importance of subunit vaccine antigen of major Fli C antigenic site of Salmonella Enteritidis II: A challenge trial
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Importance of subunit vaccine antigen of major Fli C antigenic site of Salmonella Enteritidis II: A challenge trial

机译：肠炎沙门氏菌II的主要Fli C抗原位点亚单位疫苗抗原的重要性：一项挑战性试验

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Salmonella enterica subsp. enterica serovar Enteritidis (SE) infection in chickens shows a mild pathogenicity except for young ages, compared with other animals, and laying hens sometimes produce SE-contaminated eggs leading to public health concerns. To reduce the problem, SE bacterin in poultry farms has been applied. We previously demonstrated that a subunit antigen, g.m. part polypeptide in SE-Fli C (SEp 9), could be a candidate subunit antigen of SE vaccine which may show less side effects in chickens. In this study, we used SEp 9 along with an adjuvant to inoculate chickens, then the chickens were orally challenged with SE, and suppression of the SE count in the cecum was investigated. Chickens inoculated with a commercial SE vaccine were prepared as positive controls (vaccine group), and those with physiological saline (control group) for comparison of the bacterial count after challenge. Employing two types of antibody-detection ELISA coated with either de-flagellated SE or SEp 9, specific antibody levels in blood and the intestine were determined. The bacterial count was significantly lower 1 and 3 weeks after challenge in the SEp 9 than in the control group. Specific antibody only against SEp 9 in blood but not the intestine of these birds in the SEp 9 group was detected. This study confirmed that SEp 9 antigen is a major effective antigen in SE inactivated vaccine, and it is suggested that only the subunit vaccine antigen SEp 9 is needed to effectively suppress colonization in the chicken intestine, without the need for other SE component antigens.
机译：肠沙门氏菌亚种。与其他动物相比，鸡中的肠型肠炎沙门氏菌（SE）感染除年龄较小外均表现出轻度的致病性，产蛋鸡有时会产生被SE污染的卵，从而引起公众健康问题。为了减少该问题，已在家禽农场中使用了SE细菌。我们先前证明了亚单位抗原，例如g.m。 SE-Fli C中的部分多肽（SEp 9），可能是SE疫苗的候选亚基抗原，可能在鸡中显示出较小的副作用。在这项研究中，我们将SEp 9与佐剂一起接种给鸡，然后对鸡进行SE口服攻击，并研究了盲肠中SE计数的抑制作用。制备接种商业SE疫苗的鸡作为阳性对照（疫苗组），并接种生理盐水的鸡（对照组）作为激发后细菌计数的比较。采用两种类型的抗体检测ELISA包被了脱鞭毛的SE或SEp 9，确定了血液和肠道中的特异性抗体水平。 SEp 9攻击后1周和3周，细菌计数显着低于对照组。仅检测到针对血液中SEp 9的特异性抗体，而未检测到SEp 9组中这些鸟类的肠道。这项研究证实SEp 9抗原是SE灭活疫苗中的主要有效抗原，并且建议仅需要亚单位疫苗抗原SEp 9即可有效抑制鸡肠内的定植，而无需其他SE成分抗原。

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