Use of MDCK cells for production of live attenuated influenza vaccine. (Special Issue: Advances in vaccine technology II 2008.)

Liu J.; Shi X.; Schwartz R.; Kemble G.

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Use of MDCK cells for production of live attenuated influenza vaccine. (Special Issue: Advances in vaccine technology II 2008.)

机译：MDCK细胞用于生产减毒活疫苗的用途。（特刊：疫苗技术的进步II，2008年。）

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To develop a cell-based live attenuated influenza vaccine (LAIV) manufacturing process, several different cell lines were evaluated by comparing the titer of viruses after infection with LAIV strains. While several cell lines have been reported to support influenza virus replication, the degree of replication and the ability to support replication of LAIV strains have not been systematically examined. MDCK cells, which have been considered as potential substrates for influenza vaccine production were evaluated in addition to Vero, MRC-5, WI-38 and FRhL cells. MRC-5, WI-38 and FRhL cells produced low to moderate titers of virus with titers equal or below 5.0 log₁₀ TCID₅₀/mL. Both Vero and MDCK cells could support a higher level of virus replication for certain strains, however, Vero cells only produced high titers when grown in the presence of serum. MDCK cells supported high levels of vaccine virus production for multiple different LAIV subtypes in both serum containing and serum-free media. These results suggest that MDCK cell-based production can be used as an alternative production platform to the currently used egg-based LAIV production system.

机译：为了开发基于细胞的减毒活流感疫苗（LAIV）的生产工艺，通过比较感染LAIV株后的病毒效价，评估了几种不同的细胞系。虽然已经报道了几种支持流感病毒复制的细胞系，但是尚未系统地检查复制程度和支持LAIV病毒株复制的能力。除了Vero，MRC-5，WI-38和FRhL细胞外，还评估了被认为是生产流感疫苗的潜在底物的MDCK细胞。 MRC-5，WI-38和FRhL细胞产生的病毒滴度低至中等，滴度等于或低于5.0 log _{10 TCID _{50 / mL。对于某些菌株，Vero和MDCK细胞都可以支持更高水平的病毒复制，但是，Vero细胞仅在存在血清的情况下生长时才产生高滴度。 MDCK细胞在含血清和无血清培养基中支持多种不同LAIV亚型的高水平疫苗病毒生产。这些结果表明，基于MDCK细胞的生产可以用作当前使用的基于鸡蛋的LAIV生产系统的替代生产平台。}}

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《Vaccine》 |2009年第46期|共4页
作者
Liu J.; Shi X.; Schwartz R.; Kemble G.;
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正文语种 eng
中图分类预防医学、卫生学;
关键词
cell lines; immunization; influenza; Influenza viruses; live vaccines; replication; strains; vaccination; vaccine development;

机译：细胞系;免疫;流行性感冒;流行性感冒病毒;活疫苗;复制;菌株;疫苗接种;疫苗发育;

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1. Use of MDCK cells for production of live attenuated influenza vaccine. (Special Issue: Advances in vaccine technology II 2008.) [J] . Liu J., Shi X., Schwartz R., Vaccine . 2009,第46期

机译：MDCK细胞用于生产减毒活疫苗的用途。（特刊：疫苗技术的进步II，2008年。）
2. Suspension-Vero cell cultures as a platform for viral vaccine production. (Special Issue: Advances in vaccine technology II 2008.) [J] . Paillet C., Forno G., Kratje R., Vaccine . 2009,第46期

机译：悬浮病毒细胞培养作为生产病毒疫苗的平台。（特刊：疫苗技术的进步II，2008年。）
3. VaxfectinReg., a cationic lipid-based adjuvant for protein-based influenza vaccines. (Special Issue: Advances in vaccine technology II 2008.) [J] . Hartikka J., Bozoukova V., Yang C. K., Vaccine . 2009,第46期

机译：VaxfectinReg。，一种用于蛋白质型流感疫苗的基于阳离子脂质的佐剂。（特刊：疫苗技术的进步II，2008年。）
4. Influence of Host Cell Defence during Influenza Vaccine Production in MDCK Cells - (PPT) [C] . T. Frensing, C. Seitz, B. Heynisch, Vaccine Technology Symposium . 2010

机译：MDCK细胞流感疫苗生产过程中宿主细胞防御的影响 - （PPT）
5. Preservation of two therapeutic biopharmaceuticals using sugars and polymers: Hematopoietic stem and progenitor cells and a live attenuated viral vaccine. [D] . Buchanan, Sandhya S. 2006

机译：使用糖和聚合物保存两种治疗性生物药物：造血干细胞和祖细胞以及减毒活疫苗。
6. Comparison of egg and high yielding MDCK cell-derived live attenuated influenza virus for commercial production of trivalent influenza vaccine: In vitro cell susceptibility and influenza virus replication kinetics in permissive and semi-permissive cells [O] . Althaf I. Hussain, Melissa Cordeiro, Elizabeth Sevilla, -1

机译：商业生产三价流感疫苗的蛋和高产MDCK细胞减毒活流感病毒的比较：允许和半允许细胞中的体外细胞敏感性和流感病毒复制动力学
7. Comparison of egg and high yielding MDCK cell-derived live attenuated influenza virus for commercial production of trivalent influenza vaccine: In vitro cell susceptibility and influenza virus replication kinetics in permissive and semi-permissive cells [O] . Althaf I. Hussain, Melissa Cordeiro, Elizabeth Sevilla, 2010

机译：卵子和高产MDCK细胞衍生实时病毒的比较流感疫苗商业生产的流感病毒：在允许和半允许细胞中的体外细胞敏感性和流感病毒复制动力学
8. Comparison of the Effectiveness of Trivalent Inactivated Influenza Vaccine and Live, Attenuated Influenza Vaccine in Preventing Influenza-Like Illness among US Service Members, 2006-2009. [R] . Phillips, C. J., Woolpert, T., Sevick, C., 2012

机译：2006 - 2009年美国服务成员中三价灭活流感疫苗和活减毒流感疫苗预防流感样疾病的效果比较。

Use of MDCK cells for production of live attenuated influenza vaccine. (Special Issue: Advances in vaccine technology II 2008.)

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