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首页> 外文期刊>Vaccine >Altered pathogenicity, immunogenicity, tissue tropism and 3 '-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
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Altered pathogenicity, immunogenicity, tissue tropism and 3 '-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos

机译：禽类传染性支气管炎冠状病毒株在胚胎中连续传代后的致病性，免疫原性，组织嗜性和3'-7 kb区域序列的改变

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摘要

In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7 kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mixture of genetic mutants. The titers of the virus increased gradually during serial passage, but the replication capacity decreased in chickens. The virus was partially attenuated at passage 40 (P40) and P70, and was fully attenuated at P110. It lost immunogenicity and kidney tropism at P110 and P70, respectively. Amino acid substitutions were found in the 3'-7 kb region. primarily in the spike (S) protein. Substitutions in the S1 subunit occurred between P3 and P40 and all subpopulations in a virus passage showed the same substitutions. Other substitutions that occurred between P70 and P110, however, were found only in some subpopulations of the virus passages. A 109-bp deletion in the 3'-UTR was observed in most subpopulations of P70 and P110, and might be related to virus replication, transcription and pathogenicity. The changes described in the 3'-7 kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes.
机译：在这项研究中，我们通过在鸡胚卵中进行连续传代来减弱中国LX4型肾病性传染性支气管炎病毒（IBV）株CK / CH / LHLJ / 04V。根据3'-7 kb区域的序列分析，CK / CH / LHLJ / 04V病毒种群包含带有遗传突变体混合物的亚群。该病毒的滴度在连续传代过程中逐渐增加，但在鸡中的复制能力下降。该病毒在第40代（P40）和P70处部分减毒，并在P110处完全减毒。它分别在P110和P70失去了免疫原性和肾脏嗜性。在3'-7 kb区域发现了氨基酸取代。主要在刺突（S）蛋白中。 S1亚基的取代发生在P3和P40之间，病毒传代中的所有亚群都显示相同的取代。但是，仅在病毒传代的某些亚群中发现了在P70和P110之间发生的其他取代。在P70和P110的大多数亚群中，在3'-UTR中删除了109 bp，这可能与病毒复制，转录和致病性有关。病毒3'-7 kb区域中描述的变化可能导致病毒减毒，免疫原性降低和组织嗜性变化。但是，我们不能排除基因组其他部分也可能参与这些变化的可能性。

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