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首页> 外文期刊>Vaccine >Identification of protective antigens by RNA interference for control of the lone star tick, Amblyomma americanum.
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Identification of protective antigens by RNA interference for control of the lone star tick, Amblyomma americanum.

机译：通过RNA干扰鉴定保护性抗原，以控制孤星tick（i）American Amblyomma americanum 。

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The lone star tick, Amblyomma americanum, vectors pathogens of emerging diseases of humans and animals in the United States. Currently, measures are not available for effective control of A. americanum infestations. Development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. However, the limiting step in tick vaccine development has been the identification of tick protective antigens. Herein, we report the application of RNA interference (RNAi) for screening an A. americanum cDNA library for discovery of tick protective antigens that reduce tick survival and weights after feeding. Four cDNA clones, encoding for putative threonyl-tRNA synthetase (2C9), 60S ribosomal proteins L13a (2D10) and L13e (2B7), and interphase cytoplasm foci protein 45 (2G7), were selected for vaccine studies in cattle, along with subolesin, a tick protective protein identified previously. In vaccinated cattle, an overall efficacy (E) >30% was obtained when considering the vaccine effect on both nymphs and adults, but only 2D10, 2G7 and subolesin affected both tick stages. The highest efficacy of control for adult ticks (E
机译：孤星tick在美国是人类和动物新发疾病的病原体。当前，尚无有效控制A的措施。美洲出没。针对tick蛋白的疫苗的开发可以减少tick的侵扰和and传播的病原体的传播。但是，壁虱疫苗开发的限制步骤是鉴定壁虱保护性抗原。在此，我们报道了RNA干扰（RNAi）在筛选iA中的应用。 americanum cDNA文库，用于发现壁虱保护性抗原，这些抗原可降低壁虱的存活率并减少其摄食后的体重。选择了四个编码推定的苏氨酰-tRNA合成酶（2C9），60S核糖体蛋白L13a（2D10）和L13e（2B7）和间质细胞质病灶蛋白45（2G7）的cDNA克隆以及牛油蛋白，用于牛的疫苗研究。先前鉴定的保护蛋白。当考虑到疫苗对若虫和成虫的影响时，在接种牛中，总功效（ E ）> 30％，但是只有2D10、2G7和油质松脂素同时影响两个tick虫阶段。在接种重组2G7或亚油精的牛中获得了最高的成年s虱防治效果（ E

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