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首页> 外文期刊>Vaccine >DNA vaccination with asparaginyl endopeptidase (Sm32) from the parasite Schistosoma mansoni: anti-fecundity effect induced in mice
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DNA vaccination with asparaginyl endopeptidase (Sm32) from the parasite Schistosoma mansoni: anti-fecundity effect induced in mice

机译：曼氏血吸虫寄生虫的天冬酰胺基内肽酶（Sm32）的DNA疫苗接种：在小鼠中诱导的抗生殖力作用

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摘要

DNA-based vaccine technology was used to induce an immune response in mice against a schistosome cysteine proteinase, asparaginyl endopeptidase (Sm32). The cDNA coding for Sm32 was cloned in a mammalian expression vector under control of the CMV promoter/enhancer and expressed for the first time in transfected mammalian cells as well as in mice immunized with the Sm32-encoding DNA construct. These mice developed antibodies which recognized the native protein not only in homogenates of Schistosoma mansoni worms but also in the gut on cryostat sections of the parasites. This DNA vaccine led to an anti-fecundity effect: female worms of a challenge infection produced 37% less eggs than those growing in naive mice. The results suggest that Sm32 may be a candidate antigen for the generation of an anti-pathology vaccine against schistosomes.
机译：基于DNA的疫苗技术被用于诱导小鼠针对血吸虫半胱氨酸蛋白酶，天冬酰胺基内肽酶（Sm32）的免疫反应。在CMV启动子/增强子的控制下，将编码Sm32的cDNA克隆到哺乳动物表达载体中，并在转染的哺乳动物细胞中以及在用编码Sm32的DNA构建体免疫的小鼠中首次表达。这些小鼠产生的抗体不仅可以识别曼氏血吸虫蠕虫的匀浆中的蛋白质，而且还可以识别寄生虫的低温恒温器切片中的肠道中的天然蛋白质。这种DNA疫苗具有抗生殖力作用：挑战感染的雌性蠕虫产生的卵比幼稚小鼠中生长的卵少37％。结果表明，Sm32可能是针对血吸虫的抗病理疫苗的候选抗原。

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