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首页> 外文期刊>Vaccine >Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters
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Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters

机译：钩端螺旋体免疫球蛋白样蛋白A（LigA）DNA疫苗对仓鼠攻击的保护性免疫评估

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摘要

We demonstrated earlier that immunization with recombinant Leptospira immunoglobulin like protein A (LigA) induced significant protection against virulent Leptospira interrogans serovar Pomona challenge in hamsters. However, the protective immune mechanism remains unclear. In the present study we demonstrated the protective efficacy of a LigA DNA vaccine and evaluated the immune mechanism underlying the protection against leptospirosis in hamsters. The LigA DNA vaccine was constructed in two truncated forms as the conserved portion (LigAcon) and a variable portion (LigAvar). Four-week-old hamsters were immunized three times at two-week intervals with vector alone or an equal amount of a recombinant construct containing either LigAcon or LigAvar. All animals were challenged intraperitoneally 2 weeks after the last immunization with a dose (LD(50)=10(8)) of virulent L. interrogans serovar Pomona. Prior to challenge, four animals were sacrificed, the spleen was removed aseptically, and splenocytes were assayed for lymphocyte proliferation and cytokine profiles in response to recall antigen. The protective efficacy was evaluated on the basis of survival and histopathological lesions in the kidney. The immuno-protective mechanism was assessed on the basis of Th1/Th2 profile of cytokines in immunized animals. Our results indicate that immunization with LigA DNA vaccine provides significant protection against leptospirosis. We suggest that immuno-protection is conferred by both humoral and cellular immunity as revealed by an increase in antibody titers during subsequent boosters, significant proliferation of lymphocytes and enhancement of both Th1 and Th2 cytokines. Taken together, the present study suggests that a LigA DNA vaccine is a promising candidate for prevention of leptospirosis.
机译：我们较早地证明，用重组钩端螺旋体免疫球蛋白（如蛋白A（LigA））进行免疫接种可显着保护仓鼠抵抗强毒钩端螺旋体血清型Pomona攻击。但是，保护性免疫机制仍不清楚。在本研究中，我们证明了LigA DNA疫苗的保护功效，并评估了针对仓鼠钩端螺旋体病的保护作用的免疫机制。 LigA DNA疫苗以两种截短形式构建，分别是保守部分（LigAcon）和可变部分（LigAvar）。用单独的载体或等量的含有LigAcon或LigAvar的重组构建体，以两周的间隔免疫四周龄的仓鼠3次。在最后一次免疫接种后2周，用强力的问号L. interrogans serovar Pomona剂量（LD（50）= 10（8））腹膜内攻击所有动物。在攻击之前，处死四只动物，无菌除去脾脏，并测定脾细胞响应于召回抗原的淋巴细胞增殖和细胞因子谱。根据存活率和肾脏的组织病理学损害评估保护效果。基于免疫动物中细胞因子的Th1 / Th2谱评估免疫保护机制。我们的结果表明，用LigA DNA疫苗免疫可有效预防钩端螺旋体病。我们建议通过体液免疫和细胞免疫赋予免疫保护，如在随后的加强免疫期间抗体滴度的增加，淋巴细胞的显着增殖以及Th1和Th2细胞因子的增强所揭示。综上所述，本研究表明，LigA DNA疫苗是预防钩端螺旋体病的有前途的候选药物。

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