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首页> 外文期刊>Vaccine >Timely production of A/Fujian-like influenza vaccine matching the 2003-2004 epidemic strain may have been possible using Madin-Darby canine kidney cells
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Timely production of A/Fujian-like influenza vaccine matching the 2003-2004 epidemic strain may have been possible using Madin-Darby canine kidney cells

机译：使用Madin-Darby犬肾细胞可能有可能及时生产出与2003-2004年流行株匹配的A /福建样流感疫苗

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摘要

Timely production and antigenic match with those of the epidemic strains are required for influenza vaccines. A/Fujian/411/2002-like (H3N2) virus Was the main epidemic influenza Virus during the 2003/2004 season in the northern hemisphere. But A/Fujian-like reassortant viruses were not available until more than one year later. We evaluated the A/Kumamoto/102/2002 strain, ail A/Fujian/411/2002-like strain isolated in 2002, as a potential vaccine. We compared A/Kumamoto/102/2002 viruses isolated from the same clinical sample in Madin-Darby canine kidney (MDCK) cells and eggs. Kumamoto/102/2002 isolated from eggs grew poorly and showed amino acid Mutations of haemagglutinin. In contrast, A/Kumamoto/102/2002 isolated from MDCK cells grew well in MDCK suspension culture. The amino acid sequence of MDCK-derived A/Kumamoto virus was identical to that of A/Fujian/411/2002. These results suggest that culture in MDCK cells could have produced ail influenza vaccine with a better antigenetic match to the predicted epidemic strain for the 2003/2004 season than the vaccine actually produced. (C) 2008 Elsevier Ltd. All rights reserved.
机译：流感疫苗需要及时生产并与流行株进行抗原匹配。 A / Fujian / 411 / 2002-like（H3N2）病毒是北半球2003/2004季节的主要流行流感病毒。但是直到一年多以后，才出现了类似A /福建的重组病毒。我们评估了A / Kumamoto / 102/2002菌株，所有2002年分离的A / Fujian / 411/2002样菌株，作为一种潜在的疫苗。我们比较了从同一临床样本中分离的Madin-Darby犬肾（MDCK）细胞和卵中的A / Kumamoto / 102/2002病毒。从鸡蛋中分离出的熊本/ 102/2002生长缓慢，并显示出血凝素的氨基酸突变。相反，从MDCK细胞分离的A / Kumamoto / 102/2002在MDCK悬浮培养中生长良好。 MDCK来源的A /熊本病毒的氨基酸序列与A / Fujian / 411/2002的相同。这些结果表明，与实际生产的疫苗相比，在MDCK细胞中培养的所有流感疫苗在2003/2004季与预期的流行株的抗原匹配都更好。 （C）2008 Elsevier Ltd.保留所有权利。

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