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首页> 外文期刊>Vaccine >Stabilisation of Salmonella vaccine vectors by the induction of trehalosebiosynthesis
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Stabilisation of Salmonella vaccine vectors by the induction of trehalosebiosynthesis

机译：通过诱导海藻糖合成来稳定沙门氏菌疫苗载体

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The growth of an aroA mutant of Salmonella typhimurium (SL3261) in minimal medium containing 0.5 M NaCl resulted in the intracellular accumulation of 2.2 mu mol trehalose/mg total protein. The vacuum drying of these bacteria in the presence of trehalose allowed the recovery of 35% of the viable cells that were present before drying. In contrast, bacteria cultured in control medium accumulated 0.4 mu mol trehalose/mg total protein and only 5% of the viable cells were recovered after vacuum drying with trehalose. Similar results were obtained when S. typhimurium SL3261, expressing the vaccine antigen (F1-antigen) of Yersinia pestis, was cultured in minimal medium with or without 0.5 M NaCl and dried in the presence of trehalose. Although these results indicate the potential for trehalose stabilisation of vaccine strains of S. typhimiurium, growth in minimal medium containing 0.5 M NaCl resulted in the loss of invasion competence of the bacteria.
机译：鼠伤寒沙门氏菌（SL3261）的aroA突变体在含有0.5 M NaCl的基本培养基中的生长导致细胞内积累2.2μmol海藻糖/ mg总蛋白。在海藻糖存在下对这些细菌进行真空干燥，可以回收干燥前存在的35％的活细胞。相反，在对照培养基中培养的细菌积累了0.4μmol海藻糖/ mg总蛋白，用海藻糖真空干燥后仅回收了5％的活细胞。当表达鼠疫耶尔森氏菌的疫苗抗原（F1-抗原）的鼠伤寒沙门氏菌SL3261在含有或不含0.5 M NaCl的基本培养基中培养并在海藻糖存在下干燥时，可获得相似的结果。尽管这些结果表明了鼠伤寒沙门氏菌疫苗菌株海藻糖稳定化的潜力，但在含有0.5 M NaCl的基本培养基中的生长导致细菌入侵能力的丧失。

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