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首页> 外文期刊>Vaccine >A proteoliposome based formulation administered by the nasal route produces vibriocidal antibodies against el Tor Ogawa Vibrio cholerae O1 in BALB/c mice
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A proteoliposome based formulation administered by the nasal route produces vibriocidal antibodies against el Tor Ogawa Vibrio cholerae O1 in BALB/c mice

机译：通过鼻腔途径给药的基于蛋白脂质体的制剂在BALB / c小鼠中产生抗el Tor Ogawa霍乱弧菌O1的杀弧菌抗体

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摘要

A vaccine candidate against the enteric pathogen Vibrio cholerae was developed based on a proteoliposome (PL) formulation using a wild type strain C7258, V. cholerae O1, el Tor Ogawa as part of strategy to develop a combined formulation against enteric diseases preventable by the stimulation of the mucosal immune system. A detergent extraction method was applied to obtain the PL. Scanning electron microscopy and molecular exclusion chromatography showed the presence of two PL populations. Photon correlation spectroscopy studies were then carried out to evaluate the size (169.27 +/- 3.85 nm), polydispersity (0.410) and zeta potential (-23.28 +/- 1.21 mV) of the PL. SDS-PAGE and Western blot analysis revealed the presence of lipopolysaccharide (LPS), mannose-sensitive haemagglutinin (MSHA) and a range of outer membrane proteins, including OmpU. BALB/c mice were immunized intranasally with two doses of PL containing 25 mu g of LPS each 28 days apart. The mice showed high anti-LPS IgG titres (3.36 +/- 0.235) and vibriocidal antibodies (3.70 +/- 0.23) after two weeks from last dose. These results show for the first time that PL can be obtained from V. cholerae O1 and when administer by intranasal route has the potential to protect against this pathogen.
机译：基于蛋白脂质体（PL）配方，使用野生型菌株C7258，霍乱弧菌O1，霍乱tor Ogawa开发了针对肠道病原体霍乱弧菌的候选疫苗，作为开发针对可通过刺激预防的肠道疾病的联合制剂的策略的一部分黏膜免疫系统应用去污剂提取方法获得PL。扫描电子显微镜和分子排阻色谱显示存在两个PL群体。然后进行光子相关光谱研究，以评估PL的尺寸（169.27 +/- 3.85 nm），多分散性（0.410）和zeta电位（-23.28 +/- 1.21 mV）。 SDS-PAGE和Western blot分析显示存在脂多糖（LPS），甘露糖敏感的血凝素（MSHA）和一系列外膜蛋白，包括OmpU。 BALB / c小鼠经鼻内免疫，每次间隔28天，分别用两剂含25μgLPS的PL进行鼻内免疫。从最后一次给药后两周，小鼠显示出高的抗-LPS IgG滴度（3.36 +/- 0.235）和杀线虫抗体（3.70 +/- 0.23）。这些结果首次表明，可以从霍乱弧菌O1中获得PL，并且当通过鼻内途径施用时，有可能防御这种病原体。

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