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A novel virus-like particle based on hepatitis B core antigen and substrate-binding domain of bacterial molecular chaperone DnaK

机译：基于乙型肝炎核心抗原和细菌分子伴侣DnaK的底物结合结构域的新型病毒样颗粒

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Hepatitis B virus core (HBc) protein has been proved to be an attractive carrier for foreign epitopes, and can display green fluorescent protein (GFP) on its surface, The structure of substrate-binding domain of DnaK [DnaK (394-504 aa), DnaK SBD] is similar to GFP, we therefore reasoned that DnaK SBD might also be tolerated. Electron microscopic observations suggested that the chimeric proteins containing the truncated HBc (HBc Delta) and DnaK SBD could self-assemble into virus-like particle (VLP). Then the accessibility of DnaK SBD and the adjuvanticity of VLP HBc Delta-SBD were demonstrated by two recombinant peptide vaccines against gonadotropin-releasing hormone (GnRH), GhM and GhMNR. The latter carries in addition the peptide motif NRLLLTG which is known to bind to DnaK and DnaK SBD. The combination of VLP HBc Delta-SBD and GhMNR elicited stronger humoral responses and caused further testicular atrophy than the combinations of VLP HBc Delta and GhMNR or VLP HBc Delta-SBD and GhM in Balb/c mice. These findings indicate VLP HBc Delta-SBD might serve as an excellent carrier for GhMNR and some other peptide vaccines. (C) 2009 Elsevier Ltd. All rights reserved.
机译：乙肝病毒核心（HBc）蛋白已被证明是外来表位的有吸引力载体，并且可以在其表面显示绿色荧光蛋白（GFP）。DnaK [DnaK（394-504 aa）的底物结合结构域[，DnaK SBD]与GFP相似，因此我们认为DnaK SBD也可以被容忍。电子显微镜观察表明，含有截短的HBc（HBc Delta）和DnaK SBD的嵌合蛋白可以自组装成病毒样颗粒（VLP）。然后通过两种针对促性腺激素释放激素（GnRH）的重组肽疫苗GhM和GhMNR证明了DnaK SBD的可及性和VLP HBc Delta-SBD的佐剂性。后者还携带已知与DnaK和DnaK SBD结合的肽基序NRLLLTG。与Balb / c小鼠中的VLP HBc Delta-SBD和GhMNR的组合相比，VLP HBc Delta-SBD和GhMNR的组合引起的体液反应更强，并引起进一步的睾丸萎缩。这些发现表明，VLP HBc Delta-SBD可以作为GhMNR和其他一些肽疫苗的优良载体。 （C）2009 Elsevier Ltd.保留所有权利。

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