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首页> 外文期刊>Vaccine >Protective immune response against methicillin resistant Staphylococcus aureus in a murine model using a DNA vaccine approach
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Protective immune response against methicillin resistant Staphylococcus aureus in a murine model using a DNA vaccine approach

机译：使用DNA疫苗方法在鼠模型中针对耐甲氧西林金黄色葡萄球菌的保护性免疫应答

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Methicillin resistant Staphylococcus aureus (MRSA) are a major pathogen responsible for serious hospital infections worldwide. These bacteria are resistant to all beta-lactam antibiotics due to the production of an additional penicillin binding protein, the PBP2a, encoded by the mecA gene, which shows low affinity for this class of antibiotics. In this study, we cloned an internal region from the transpeptidase domain from the PBP2a into a mammalian expression vector, to be used as DNA vaccine in a Murine model. After three sets of DNA vaccination, the immune response represented by antibodies against a fragment of PBP2a was evaluated by enzyme linked immunosorbent assay (ELISA), showing a significant antibody response. The antibacterial effect of the DNA vaccine was evaluated by intraperitoneal immunization and challenge with a sublethal dose of MRSA for 7 days in mice. After the challenge, the number of bacteria from kidneys from immunized and non-immunized mice were determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice). The response specificity indicates no effects against the normal PBPs from staphylococci and no effects against Gram positive rods from normal intestinal flora. Our results indicate that the immunization against the PBP2a from MRSA using a DNA vaccine approach could be used as a new strategy to efficiently fight these multiresistant bacteria.
机译：耐甲氧西林的金黄色葡萄球菌（MRSA）是导致世界范围内严重医院感染的主要病原体。这些细菌对所有β-内酰胺类抗生素都有抗性，因为它产生了由mecA基因编码的额外的青霉素结合蛋白PBP2a，对这类抗生素显示出低亲和力。在这项研究中，我们将来自PBP2a的转肽酶结构域的内部区域克隆到哺乳动物表达载体中，用作鼠模型的DNA疫苗。在三组DNA疫苗接种后，通过酶联免疫吸附测定（ELISA）评估了针对PBP2a片段的抗体所代表的免疫反应，显示出显着的抗体反应。通过腹膜内免疫和用亚致死剂量的MRSA攻击小鼠7天来评估DNA疫苗的抗菌作用。攻击后，确定来自免疫小鼠和非免疫小鼠肾脏的细菌数量。免疫小鼠的肾脏细菌感染率比阳性对照（非免疫小鼠）少1000倍。响应特异性表明，葡萄球菌对正常的PBP没有影响，正常肠道菌群对革兰氏阳性棒也没有影响。我们的结果表明，使用DNA疫苗方法从MRSA中针对PBP2a进行免疫接种可以用作有效对抗这些多耐药细菌的新策略。

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