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首页> 外文期刊>Vaccine >Antigen entrapped in the escheriosomes leads to the generation of CD4(+) helper and CD8(+) cytotoxic T cell response
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Antigen entrapped in the escheriosomes leads to the generation of CD4(+) helper and CD8(+) cytotoxic T cell response

机译：抗原包埋在抗原中导致CD4（+）辅助和CD8（+）细胞毒性T细胞反应的产生

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摘要

In previous study we demonstrated the potential of Escherichia coli (E. coli) lipid liposomes (escheriosomes) to undergo membrane-membrane fusion with cytoplasmic membrane of the target cells including professional antigen presenting cells. Our present study demonstrates that antigen encapsulated in escheriosomes could be successfully delivered simultaneously to the cytosolic as well as endosomal processing pathways of antigen presenting cells, leading to the generation of both CD4(+) T-helper and CD8(+) cytotoxic T cell response. In contrast. encapsulation of same antigen in egg phosphatidyl-choline (egg PC) liposomes, just like antigen-incomplete Freund's adjuvant (IFA) complex, has inefficient access to the cytosolic pathway of MHC I-dependent antigen presentation and failed to generate antigen-specific CD8(+) cytotoxic T cell response. However, both egg PC liposomes as well as escheriosomes-encapsulated antigen elicited strong Immoral immune response in immunized animals but antibody titre was significantly higher in the group of animals immunized with escheriosomes-encapsulated antigen. These results imply usage of liposome-based adjuvant as potential candidate vaccine capable of eliciting both cell-mediated as well as Immoral immune responses. Furthermore, antigen entrapped in escheriosomes stimulates antigen-specific CD4(+) T cell proliferation and also enhances the level of IL-2, IFN-gamma and IL-4 in the immunized animals.
机译：在先前的研究中，我们证明了大肠杆菌（E. coli）脂质脂质体（escheriosomes）与包括专业抗原呈递细胞在内的靶细胞胞质膜发生膜-膜融合的潜力。我们目前的研究表明，包裹在大肠杆菌中的抗原可以成功地同时传递到抗原呈递细胞的胞质和内体加工途径，从而导致CD4（+）T-helper和CD8（+）细胞毒性T细胞反应的产生。相反。卵磷脂酰胆碱（egg PC）脂质体中相同抗原的包封，就像抗原不完全弗氏佐剂（IFA）复合物一样，无法有效地利用MHC I依赖性抗原呈递的胞质途径，并且无法产生抗原特异性CD8（ +）细胞毒性T细胞反应。但是，卵PC脂质体和包被了衣着小体的抗原在被免疫的动物中均引起了强烈的不动免疫反应，但是在被包被了衣着小体的抗原免疫的动物中，抗体的滴度明显更高。这些结果暗示使用基于脂质体的佐剂作为能够引发细胞介导的以及不道德的免疫应答的潜在候选疫苗。此外，被包埋在大肠杆菌中的抗原刺激了抗原特异性CD4（+）T细胞增殖，并且还提高了免疫动物中IL-2，IFN-γ和IL-4的水平。

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