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首页> 外文期刊>Vaccine >CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein.
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CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein.

机译：SARS-CoV S蛋白的表位引起HLA-A * 0201转基因小鼠CD8 + T细胞应答。

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摘要

Cytotoxic CD8+ T lymphocytes (CTLs) play an important role in antiviral immunity. Several human HLA-A*0201 restricted CTL epitopes of severe acute respiratory syndrome (SARS) spike (S) protein have been identified in HLA-A*0201 transgenic (Tg) mice, but the mechanisms and properties of immune responses are still not well understood. In this study, HLA-A*0201 Tg mice were primed intramuscularly with SARS S DNA and boosted subcutaneously with HLA-A*0201 restricted peptides. The lymphocytes from draining lymph nodes, spleens and lungs were stimulated with the cognate peptides. Three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses during short and long periods of time after immunization. Results showed that peptide-specific CD8+ T cells secreted IFN- gamma , TNF- alpha and IL-2 and expressed CD107a/b on cell surface. IFN- gamma +CD8+ T cells and CD107a/b+CD8+ T cells distributed throughout the lymphoid and non-lymphoid tissues, but the frequency of peptide-specific CD8+ T cells was higher in lungs than in spleens and lymph nodes. The phenotype of the CD8+ T cells was characterized based on the expression of IFN- gamma . Most of the HLA-A*0201 restricted peptide-specific CD8+ T cells represented a memory subset with CD45RBhigh and CD62Llow. Taken together, these data demonstrate that immunization with SARS S DNA and HLA-A*0201 restricted peptides can elicit antigen-specific CD8+ T cell immune responses which may have a significant implication in the long-term protection. We provide novel information in cellular immune responses of SARS S antigen-specific CD8+ T cells, which are important in the development of vaccine against SARS-CoV infection.
机译：细胞毒性CD8 + T淋巴细胞（CTL）在抗病毒免疫中起重要作用。在HLA-A * 0201转基因（Tg）小鼠中已鉴定出几种严重急性呼吸综合征（SARS）尖峰（S）蛋白的人HLA-A * 0201限制性CTL表位，但免疫应答的机制和性质仍不理想了解。在这项研究中，用SARS S DNA肌肉注射HLA-A * 0201 Tg小鼠，并用HLA-A * 0201限制性肽皮下注射。同源肽刺激淋巴结，脾脏和肺部引流的淋巴细胞。三种不同的方法（ELISA，ELISPOT和FACS）用于评估免疫后短期和长期内的免疫反应。结果表明，肽特异性CD8 + T细胞分泌IFN-γ，TNF-α和IL-2，并在细胞表面表达CD107a / b。 IFN-γ + CD8 + T细胞和CD107a / b + CD8 + T细胞分布在整个淋巴结和非淋巴组织，但肺部的肽特异性CD8 + T细胞的频率高于脾脏和淋巴结。根据IFN-γ的表达来表征CD8 + T细胞的表型。大多数HLA-A * 0201限制性肽特异性CD8 + T细胞代表具有CD45RB high 和CD62L low 的记忆亚群。综上所述，这些数据表明用SARS S DNA和HLA-A * 0201限制性肽进行的免疫可以引起抗原特异性CD8 + T细胞免疫应答，这可能对长期保护具有重要意义。 。我们在SARS S抗原特异性CD8 + T细胞的细胞免疫应答中提供了新的信息，这对于开发抗SARS-CoV感染的疫苗具有重要意义。

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