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首页> 外文期刊>Vaccine >Protection of chickens from lethal avian influenza A virus infection bylive-virus vaccination with infectious laryngotracheitis virusrecombinants expressing the hemagglutinin (H5) gene
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Protection of chickens from lethal avian influenza A virus infection bylive-virus vaccination with infectious laryngotracheitis virusrecombinants expressing the hemagglutinin (H5) gene

机译：用表达血凝素（H5）基因的传染性喉气管炎病毒重组体进行活病毒疫苗预防鸡免受致命的甲型流感病毒感染

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摘要

The H5 hemagglutinin (HA) gene of a highly pathogenic avian influenza virus (AIV) isolate (A/chicken/Italy/8/98) was cloned and sequenced, and inserted at the non-essential UL50 (dUTPase) gene locus of a virulent strain of infectious laryngotracheitis virus (ILTV). Northern and Western blot analyses of the obtained ILTV recombinants demonstrated stable expression of the HA gene under control of the human cytomegalovirus immediate-early gene promoter. In vitro replication of the HA-expressing ILTV mutants was not affected, and infection of chickens revealed a reduced but still considerable virulence, similar to that of a UL50 gene deletion mutant without foreign gene insertion. The immunized animals produced specific antibodies against ILTV and AIV HA, and were protected against challenge infections with either virulent ILTV, or two different highly pathogenic AIV strains (A/chicken/Italy/8/98, A/chicken/Scotland/59). After challenge, no ILTV could be reisolated from protected animals, and shedding of AIV was considerably reduced. Thus, although attenuation remains to be improved, genetically engineered ILTV live-virus vaccines might be used as vectors to protect chickens also against other pathogens.
机译：克隆并测序了高致病性禽流感病毒（AIV）分离株（A / chicken / Italy / 8/98）的H5血凝素（HA）基因，并将其插入到有毒力的非必需UL50（dUTPase）基因位点传染性喉气管炎病毒（ILTV）株。对获得的ILTV重组体的Northern和Western印迹分析表明，在人巨细胞病毒立即早期基因启动子的控制下，HA基因稳定表达。表达HA的ILTV突变体的体外复制不受影响，对鸡的感染显示出降低的毒力，但仍然相当可观，类似于没有外源基因插入的UL50基因缺失突变体。免疫的动物产生针对ILTV和AIV HA的特异性抗体，并被保护免于用强力ILTV或两种不同的高致病性AIV毒株（A /鸡/意大利/ 8/98，A /鸡/苏格兰/ 59）的挑战性感染。攻击后，无法从受保护的动物中分离出ILTV，并且大大减少了AIV的脱落。因此，尽管减毒仍有待改善，但基因工程ILTV活病毒疫苗可以用作载体，以保护鸡也免受其他病原体侵害。

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